Flow Cytometry

Accurately immunophenotype cells of interest

Comprehensively Quantify Therapeutic Effects through High Throughput Flow Cytometry Analysis of Various Cell Types

Gain new insight into your drug mechanism of action and PD through robust and in-depth immunophenotyping. Maximize immunotherapeutic benefit, limit unwanted toxicities, and increase your chances of preclinical/clinical success through comprehensively characterizing cells of interest. Use our high quality flow cytometry data to better understand:

  • The immune make-up of relevant organs such as tumor, blood, spleen, and lymph nodes
  • Therapeutic effect on the frequencies and functionality of cell subsets
  • Individual specimen variations and heterogeneity of response

Characterize Complex Immune-Phenotypes and Rare Cells of Interest with our Well-Established Flow Cytometry Platform

  • Characterize cells in high throughput with limited sample volume enabled by Miltenyi gentleMACS™ Dissociators, state-of-the-art equipment including BD FACSCalibur™, BD Accuri™ C6, NovoCyte, Beckman Gallios, BD LSRFortessa™ and BD LSRFortessa X-20 (up to 17 colors) with 96-well plate HTS attachment
  • Overcome tumor complexity with our pre-optimized list of over 20 protocols for tumor tissue dissociation and parent gating strategies
  • Ensure accurate staining, processing, and gating with our stringent controls and manual compensation
  • Eliminate human error through central data processing and analysis facilitated by data transfer software
  • Gain expert guidance from our dedicated FACS team to help determine the right dissociation protocols, panel configurations, gating strategies, etc. for your study
  • Modify reports to fit evolving needs with dynamic and informative reporting packages including a variety of raw data, statistical analyses, observations, actionable recommendations, data visualization, etc.
  • Easily and securely review high quality data and reports through our cloud portal

Our standard flow cytometry panels have been validated in multiple oncology and inflammation in vivo models to provide basic to comprehensive analysis of lymphoid and myeloid cell populations. Models analyzed include syngeneics, tumor homografts (MuPrime™), human target expressing (HuGEMM™/HuCELL™), humanized, GvHD, and peritonitis models.

Customized panels are also available, which undergo thorough validation prior to running your samples. See our standard panels and gating strategy below.

Standard Murine FACS Panel for TIL Analysis

 
Markers Immune Cell Population
CD45+ Total leukocytes
CD45+ CD11b- CD3+ Total T cells
CD45+ CD11b- CD3+ CD4+ CD8+ CD4+ T helper cells
CD45+ CD11b- CD3+ CD4+ CD8+ CD8+ cytotoxic T cells
CD45+ CD11b- CD3+ CD4+ CD8+ Foxp3+ Regulatory T cells
CD45+ CD3- CD11b+ Ly-6C+ Ly-6G- M-MDSC
CD45+ CD3- CD11b+ Ly-6C- Ly-6G+ G-MDSC
CD45+ CD11b+ Ly-6C- Ly-6G- F4/80+ Macrophages
CD11b+ F4/80+ Gr-1+ MDSC
CD45+ CD11b+ Ly-6C- Ly-6G- F4/80+ IA-IEhigh CD206low/- M1 macrophages
CD45+ CD11b+ Ly-6C- Ly-6G- F4/80+ IA-IElow/- CD206+ M2 macrophages
CD45+ F4/80- Ly-6C- Ly-6G- CD3- CD335+ NK cells
CD45+ F4/80- Ly-6C- Ly-6G- CD3dim CD335+ NKT cells
CD19 B cells
PD-1 / PD-L1 Checkpoint inhibitors
Live / Dead (fixable) Live / Dead

Basic panel comprises of 10 colors
Bold markers are included in the comprehensive 16 color panel

Murine TIL Gating Strategy

 

Standard Human FACS Panel for TIL Analysis

 
Markers Immune Cell Populations
mCD45-/hCD45+ Total leukocytes
mCD45-/hCD45+/CD3+ Total T cells
mCD45-/hCD45+/CD3+/CD4+ CD4+ T helper cells
mCD45-/hCD45+/CD3+/CD8+ CD8+ cytotoxic T cells
mCD45-/hCD45+/CD3+/CD4+/Foxp3+ Regulatory T cells
mCD45-/hCD45+/CD19-/CD3-/CD56+ NK
mCD45-/hCD45+/CD19-/CD3+/CD56+ NKT
mCD45-/hCD45+/CD19-/CD3-/CD56-/CD11b+/CD68+ Macrophages
mCD45-/hCD45+/CD19-/CD3-/CD56-/HLA-DR+/CD14+CD16- Classical monocytes
mCD45-/hCD45+/CD19-/CD3-/CD56-/HLA-DR+/CD14+CD16- Intermediate monocytes
mCD45-/hCD45+/CD19-/CD3-/CD56-/HLA-DR+/CD14-CD16- Non-classical monocytes
mCD45-/hCD45+/CD19-/CD3-/CD56-/HLA-DR-/CD33+CD11b+ MDSC
mCD45-/hCD45+/CD19-/CD3-/CD56-/HLA-DR-/CD33+CD11b+/CD14+/CD15- M-MDSC
mCD45-/hCD45+/CD19-/CD3-/CD56-/HLA-DR-/CD33+CD11b+/CD14-/CD15+ P-MDSC
Live / Dead (fixable) Live / Dead

Humanized Mouse TIL Gating Strategy

 

Investigate Mechanism of Action with Functional Assays

 

Characterize the biological responses of immune cells through flow cytometry based functional assays including receptor occupancy, apoptosis, and proliferation assays.

 

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