
Orthotopic tumor models provide a clinically relevant, organ-specific tumor microenvironment (TME) to improve preclinical predictivity over subcutaneous alternatives. Generated by seeding tumor cells at the organ-specific site of the original tumor, orthotopic models mimic the primary lesion and establish better tumor, stroma, and immune component interactions than subcutaneous implantation.
This allows more human-disease relevant tumor development and progression, including spontaneous metastasis, recapitulating late-stage tumors. Orthotopic models therefore provide the ideal setting to:
Orthotopic metastatic models are most powerful when the disease is tracked in vivo in real time by optical imaging of luciferase-labeled tumor cells.
Figure 1: Luciferase-labeled model establishment by lentiviral transduction
Our collection of luciferase-labeled validated in vivo models allow you to non-invasively monitor the longitudinal growth of a tumor and to track tumor response to your treatment in real time, saving time and resources.
Our cutting edge imaging techniques and platforms include:
Our panel of orthotopic bioluminescent models include syngeneic as well as xenograft lines.
Table 1: Available bioluminescent models (additional models under development and can be developed upon client request)
Cancer Type | human Cell Line (implantation site/route) | Mouse cell line (implantation site/route) |
---|---|---|
Brain | U-87 MG-luc | |
Breast | MDA-MB-231-luc* (MFP), MCF-7-luc (MFP) |
4T1-luc (MFP with mets to lung) |
Hepatoma | HEP 3B-luc | H22-luc, Hepa 1-6-luc |
Lung | A549-luc (i.v.) | |
Multiple Myeloma | MM.1S-luc (i.tb), RPMI822-luc (i.tb.) |
|
Ovarian | A2780-luc (i.p.), SK-OV-3-luc (i.p.) |
|
Prostate | PC-3M-luc* |
Cancer Type | Human Cell Line (implantation site/route) | Mouse cell line (implantation site/route) |
---|---|---|
Leukemia | MV-4-11-luc (i.v.), Nalm6-luc (i.v.) |
|
Lymphoma | Raji-luc (i.v.), Raji-PD-L1-luc (i.v.) |
|
Multiple Myeloma | M.1S-luc (i.v.) |
Cancer Type | Human Cell Line (implantation site/route) | Mouse cell line (implantation site/route) |
---|---|---|
Lung | A549-luc (i.v.) | |
Breast | MDA-MB-231-luc* (i.c.) |
*Client license required
Models provided by sponsor but the right of use has been granted (not fit for CFDA studies)
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