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Next Generation Mass Spectrometry-based Proteomics Services

The Power of Ion Mobility and Data Independent Acquisition Strategy

Crown Bioscience offers next-generation ion mobility mass spectrometry (MS)-based proteomics services to global clients.

This will allow clients to assess the biological function of proteins for understanding signaling mechanisms within cells as well as specific biomarkers to diseases, and systematically assess quantitative differences in protein profiles of distinct samples for biomedical and clinical research.

Services Offered

protein post translational modifications-teal

Post-translational
modification analysis

Post-translational modification analysis and bundle service options are available. Please contact us for details.

Applications:

  • Global proteomics profiling of cells or tissues, with/without treatment
  • Proteomics biomarker discovery and validation
  • Drug mechanism of action and toxicity studies
  • Disease mechanism studies
  • Target identification and validation
  • Complementary analysis and correlation approaches for other omics analysis

Our Advantages

  • Better reproducibility:
    No missing values, reduced batch effect, better data parallelism, and traceability

  • Increased sensitivity and wider dynamic range, improved detection depth:
    Identify more proteins from same or lower amount of sample, significantly improved quantification especially for low abundant proteins

  • Lower amount of samples required

  • Suitable for large sample cohort studies

  • Proteomic biomarker discovery service
    Customized bioinformatic analysis available for large cohort studies

Why proteomics is important

 

Most diseases are manifested at the level of protein activity. Proteomics seeks to directly correlate the involvement of specific proteins, protein complexes and their modification status in disease states. The level of transcription of a gene gives only a rough estimate of its level of expression into a protein, and moreover, it cannot reflect the protein post transcription modifications which profoundly affect their activities.

Liquid chromatography (LC) coupled with mass spectrometry (MS) has become the gold standard in various omics fields. However, the proteome is highly complex with a wide dynamic range, and the coverage of proteomes in complex biological samples remains challenging due to the limited speed, sensitivity, and resolution of current mass spectrometers.

Factors to consider when choosing MS-based proteomics services

 

Liquid chromatography (LC) coupled with mass spectrometry has become the gold standard in various omics fields. When choosing MS-based proteomic services, there are several factors to consider:

  • Data completeness and reproducibility
  • Sensitivity and identification depth:
    • Number of identified and quantified proteins
    • Low abundant protein identification and quantification
    • Sample amount requirement
  • Speed, throughput, robustness and cost
  • Data analysis

State-of-Art Mass Spectrometry Core Facility

  • 20 top mass spectrometers such as Bruker timsTOF Pro/ timsTOF Pro 2 series, and Thermo Fisher Scientific Orbitrap Exploris™ 480
  • Additional separation dimension by ion mobility separation to traditional LC-MS/MS based proteomics
    • Bruker Trapped Ion Mobility Spectrometry (TIMS)
    • Thermo Fisher Scientific FAIMS
  • Unique diaPASEF technology to dramatically increase data acquisition speed
  • Evosep One provides robust and excellent chromatographic separation
  • Continuous analysis of thousands of samples to provide stable performance without cleaning, enhancing the stability of batch analysis

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Publication

Mouse Stromal Cells Confound Proteomic Characterization and Quantification of Xenograft Models

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Factsheet

Next Generation Ion Mobility Mass Spectrometry-based Proteomics Services

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Poster

Systematic Evaluation of Label-Free Protein Quantification Pipelines (DDA vs DIA)

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