Binchen Mao, Kaijie Xiao, Xiaobo Chen, Jun Zhu, Hongbo Gu, Sheng Guo
Label-free proteomics quantification technology powered by LC-MS/MS is now routine to characterize and quantify thousands of proteins in tumor samples in a timely manner.
Data-dependent acquisition (DDA) has been the workhorse for bottom-up proteomics during the past few years. However, DDA suffers from a high percentage of missing data originating from its stochastic nature.
Due to this, data-independent acquisition (DIA) has gained increasing popularity in the field of bottom-up proteomics, due to higher reproducibility and improved data completeness.