Next Generation Sequencing Services

Our RNA-Seq platforms are compatible with a variety of sample types, including cell lines, snap-frozen tissue, whole peripheral blood, FFPE blocks and sections, peripheral blood mononuclear cells (PBMCs), total RNA, and biopsy tissue. Compatible library preparation kits include non-Illumina for mRNA, Illumina Truseq for mRNA, and ribosomal RNA-seq for mRNA, lncRNA, and microRNA. 

Applications: 

• Differential gene expression 
• Functional annotation, pathway and network analysis 
• Driver mutation prediction 
• Tumor purity analysis 
• Molecular subtyping analysis 
• Comparative analysis with published human tumor samples upon request 
• Options to accommodate other analyse
  
  

Our WES service is suitable for sequencing-based analysis of cell lines, snap-frozen tissue, whole peripheral blood, FFPE blocks and sections, PBMCs, biopsy tissue, and mouth swabs. 

Applications: 

• Identify genomic variants on DNA level, such as gene mutation, and copy number variants 
• Co-expression network analysis 
• Comparative analysis with published human tumor samples upon request 
• Options to accommodate other analyses 
  
  

Our single cell sequencing service is suitable for analysis of cell lines, fresh tissue, whole peripheral blood, and PBMCs in single cell RNA-Seq and T cell receptor (TCR) sequencing assays 

Applications: 

• Characterize and identify heterogeneous cell populations 
• Discover new cell markers and regulatory pathways 
• Uncover novel cell types, cell states & rare cell types 
• Reconstruct developmental hierarchies and reveal lineage relationships 
• Profiling healthy and diseased tissue and organs 

We also offer bioinformatics analysis for our single cell sequencing service, which includes: 

• QC and cell/gene filtering 
• Cluster analysis on integrated samples 
• Exploration of known cell type markers 
• Identification of cluster-specific markers 
• Functional analysis on cluster-specific markers 
• Further customized analysis upon request 
  
  

Crown Bioscience uses the PacBio Sequel II platform, which offers single-molecule, real-time (SMRT) sequencing. Long reads are readily assembled into complete genomes for sequencing of full-length transcripts with >99.999% consensus accuracy. Other benefits include uniform coverage of typically inaccessible regions, single-molecule resolution, and direct detection of epigenetic modifications. 

Applications: 

• WGS—de novo assembly, variant detection, and structural variant detection 
• RNA sequencing—full-length transcripts for whole transcriptome and genome annotation 
• Complex populations—full-length 16S, metagenomic functional profiling, and shotgun metagenomic assembly 
  
  

Unrivaled Structural Variant Detection with Bionano Genomics Saphyr^® Platform

Large structural variations are responsible for many diseases and conditions, including cancers and developmental disorders. Optical genome mapping with Saphyr detects structural variations ranging from 500 bp to megabase pairs in length and offers assembly and discovery algorithms that far outperform sequencing-based technologies in sensitivity.

• The Bionano Saphyr^® System detects structural variations in an unbiased manner at much higher sensitivities than sequencing-based technologies, and routinely at 5% variant allele fraction
  
  
• Complementing OGM with sequencing can reveal more answers to challenging genomic questions in genetic diseases and cancer research

Key Applications:

• For cancer research:
  • Genome variation common to cancer is too complex for low coverage whole genome sequencing
  • Complex rearrangements as well as highly repetitive regions of the genome present additional challenges for sequencing
  • For heterogeneous cancer samples, Saphyr detects all types of structural variants down to 1% variant allele fraction
• For genetic disease:
  • Find new candidate genes
  • Identify new variants in known genes
  • Discover repeat expansions
  • Finds variants other technologies can’t see
• Genome assembly:
  • Reveal true long-range structure of the genome while reducing sequencing costs
  • Build-in pipeline “hybrid scaffold” could increase the accuracy of genome assembly
  • Including OGM data into de novo genome assemblies has become a gold standard
• Advance cytogenomics:
  • Replace traditional cytogenetics with convenience, higher resolution and a more complete picture of complex aberration.
  • 10,000x greater resolution compared to karyotyping
  • Consolidate traditional cytogenetic assays into a single workflow

We offer advanced microbiome sequencing services, including full-length 16S rRNA analysis, shotgun metagenomic sequencing, and metatranscriptomic sequencing, to provide a comprehensive understanding of the microbiome through genomic sequencing and analysis.

Learn More

At Crown Bioscience, we offer cutting-edge NanoString nCounter platform services to analyze preclinical and research use only (RUO) clinical samples.

Learn More