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In Vitro

Boost oncology drug discovery with XenoBase®, featuring the largest cell line selection and exclusive 3D organoid models. Benefit from OrganoidXplore™ and OmniScreen™ for rapid, in-depth analysis.

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In Vivo

Enhance drug development with our validated in vivo models, in vitro/ex vivo assays, and in silico modeling. Tailored solutions to optimize your candidates.

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Tissue

Experience ISO-certified biobanking quality. Access top biospecimens from a global clinical network, annotated by experts for precise research.

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Biomarkers and Bioanalysis

Leverage our global labs and 150+ scientists for fast, tailored project execution. Benefit from our expertise, cutting-edge tech, and validated workflows for reliable data outcomes.

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Data Science and Bioinformatics

Harness your data and discover biomarkers with our top bioinformatics expertise. Maximize data value and gain critical insights to accelerate drug discovery and elevate projects.

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KRAS

Accelerate innovative cancer treatments with our advanced models and precise drug screening for KRAS mutations, efficiently turning insights into clinical breakthroughs.

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EGFR

Advance translational pharmacology with our diverse pre-clinical models, robust assays, and data science-driven biomarker analysis, multi-omics, and spatial biology.

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Drug Resistance

Our suite integrates preclinical solutions, bioanalytical read-outs, and multi-omics to uncover drug resistance markers and expedite discovery with our unique four-step strategy.

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Patient Tissue

Enhance treatments with our human tumor and mouse models, including xenografts and organoids, for accurate cancer biology representation.

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Target to Lead Selection for ADCs and Biologics

Journey through the drug discovery pipeline from target discovery to in vivo pharmacology. Take advantage of the Largest biobank of patient-derived models, Model development capabilities, Cell-based assays for Screening ADCs, and advanced downstream analysis.

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Bioinformatics

Apply the most appropriate in silico framework to your pharmacology data or historical datasets to elevate your study design and analysis, and to improve your chances of clinical success.

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Biomarker Analysis

Integrate advanced statistics into your drug development projects to gain significant biological insight into your therapeutic candidate, with our expert team of bioinformaticians.

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CRISPR/Cas9

Accelerate your discoveries with our reliable CRISPR solutions. Our global CRISPR licenses cover an integrated drug discovery platform for in vitro and in vivo efficacy studies.

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Genomics

Rely on our experienced genomics services to deliver high quality, interpretable results using highly sensitive PCR-based, real-time PCR, and NGS technologies and advanced data analytics.

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In Vitro High Content Imaging

Gain more insights into tumor growth and disease progression by leveraging our 2D and 3D fluorescence optical imaging.

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Mass Spectrometry-based Proteomics

Next-generation ion mobility mass spectrometry (MS)-based proteomics services available globally to help meet your study needs.

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Ex Vivo Patient Tissue

Gain better insight into the phenotypic response of your therapeutic candidate in organoids and ex vivo patient tissue.

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Spatial Multi-Omics Analysis

Certified CRO services with NanoString GeoMx Digital Spatial Profiling.

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Biomarker Discovery

De-risk your drug development with early identification of candidate biomarkers and utilize our biomarker discovery services to optimize clinical trial design.

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DMPK Services

Rapidly evaluate your molecule’s pharmaceutical and safety properties with our in vivo drug metabolism and pharmacokinetic (DMPK) services to select the most robust drug formulations.

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Efficacy Testing

Explore how the novel HuGEMM™ and HuCELL™ platforms can assess the efficacy of your molecule and accelerate your immuno-oncology drug discovery programs.

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Laboratory Services

Employ cutting-edge multi-omics methods to obtain accurate and comprehensive data for optimal data-based decisions.

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Pharmacology & Bioanalytical Services

Leverage our suite of structural biology services including, recombinant protein expression and protein crystallography, and target validation services including RNAi.

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Screens

Find the most appropriate screen to accelerate your drug development: discover in vivo screens with MuScreen™ and in vitro cell line screening with OmniScreen™.

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Toxicology

Carry out safety pharmacology studies as standalone assessments or embedded within our overall toxicological profiling to assess cardiovascular, metabolic and renal/urinary systems.

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Preclinical Consulting Services

Learn more about how our consulting services can help to support your journey to the clinic.

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Our Company

Global CRO in California, USA offering preclinical and translational oncology platforms with high-quality in vivo, in vitro, and ex vivo models.

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Our Purpose

Learn more about the impact we make through our scientific talent, high-quality standards, and innovation.

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Our Responsibility

We build a sustainable future by supporting employee growth, fostering leadership, and exceeding customer needs. Our values focus on innovation, social responsibility, and community well-being.

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Meet Our Leadership Team

We build a sustainable future by fostering leadership, employee growth, and exceeding customer needs with innovation and social responsibility.

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Scientific Advisory Board

Our Scientific Advisory Board of experts shapes our strategy and ensures top scientific standards in research and development.

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News & Events

Stay updated with Crown Bioscience's latest news, achievements, and announcements. Check our schedule for upcoming events and plan your visit.

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Career Opportunities

Join us for a fast-paced career addressing life science needs with innovative technologies. Thrive in a respectful, growth-focused environment.

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Scientific Publications

Access our latest scientific research and peer-reviewed articles. Discover cutting-edge findings and insights driving innovation and excellence in bioscience.

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Resources

Discover valuable insights and curated materials to support your R&D efforts. Explore the latest trends, innovations, and expertly curated content in bioscience.

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Blogs

Explore our blogs for the latest insights, research breakthroughs, and industry trends. Stay educated with expert perspectives and in-depth articles driving innovation in bioscience.

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Target Validation

Discover. Analyze. Validate.

Identify and Validate the Function of Biological Targets

The advent of fast and reliable next generation technologies for the genomic and proteomic characterization of biological specimens has contributed to the identification of a wealth of targets that can be potentially exploited for treating cancer. However an in-depth molecular understanding of cellular processes is required to transition potential targets into pharmacological candidates.

At Crown Bioscience, we use a comprehensive tool set to determine the biological functions of each target as well as the consequences of pharmacological intervention.

In Vitro, In Vivo, and Ex Vivo techniques

Interfering RNA

The specific knockdown of a gene of interest by RNA interference (RNAi) is a powerful tool for target discovery or validation. Gene knockdown is achieved through the use of small interfering RNAs (siRNAs) for transient downregulation of the gene of interest in the cell line.

We have expertise using a wide variety of lipid-based transfection reagents to deliver siRNAs to the cells of interest. Our robust systems and processes ensure that expression of the target gene of choice and the protein it encodes is significantly reduced.

Gene Cloning and Stable Transfection

De novo or enhanced expression of genes in mammalian cells can be achieved through molecular cloning, with genes of interest cloned and overexpressed in the cells of choice for target validation.

We utilize a variety of expression systems (both plasmid- and lentiviral-based) to deliver constitutive or inducible high level expression of wild type and mutated genes (e.g. constitutively active or kinase dead mutants).

Following establishment of stable recombinant cell lines, downstream effects of gene overexpression can be monitored through in vitro or in vivo assays.

Real-Time PCR

Real-time PCR (RT-PCR) provides a quantitative comparison of gene expression in cell lines or tissues, and is used to determine whether different gene expression levels are associated with a particular cell phenotype. At Crown Bioscience we also use RT-PCR in conjunction with RNAi technology to verify changes in gene expression.

Target Protein Knockdown and Overexpression

A variety of assays, including western blot analysis, flow cytometry, immunohistochemistry, and immunofluorescence are routinely used at Crown Bioscience to determine knockdown or overexpression of the target protein of choice.

In Vivo/Ex Vivo Target Validation

Thanks to our unique collection of validated and well-characterized Cell Line Derived Xenograft tumor models, human primary cancer cells (PrimePanel™), and patient tumor samples we can correlate in vitro preclinical findings with in vivo data, which can be translated to clinical patient outcomes.

The effect of interfering with a gene function can be evaluated in vivo using tumors grown in mice as xenografts or ex vivo using tumor tissue or tumor derived cell suspensions. The activity of your targeted agent on tumor growth can be measured directly and target-specific biomarkers can be investigated using our unique models.

Reporter Gene Assays

Reporter assays enable monitoring of enhanced expression or nuclear localization of transcription factors. Binding of the transcription factor enables transcription and translation of the reporter gene, which can be quantified in vitro or in vivo by measuring light emitted in the presence of the substrate.

We provide a number of reporter gene assays to measure specific conditions in vitro or in vivo (e.g. hypoxia) in real time, or to follow tumor growth and metastasis over time.

 

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