Cell Line Authentication with Deep Sequencing

Are your cell lines and biological models REALLY what you think they are? According to multiple studies, 1/5 to 1/3 of popular cell lines have been contaminated by intraspecies/interspecies cells, mycoplasma and/or viruses. The International Cell Line Authentication Committee (ICLAC) has documented a growing list of more than 530 misidentified cell lines (update in Jan 2023) having no known authentic stock.

Ensure confidence in your models and reproducibility of high quality data by using our deep sequencing-based cell line authentication service. The panel encompasses over 2000 SNPs and chromosome segments to accurately characterize mouse and human samples including cell lines, organoids, xenograft models, and patient tissues. We have also generated unique DNA fingerprints for the most commonly used oncology research models, covering over 1,200 human cancer and 90 mouse cell lines.

Conventional PCR-based STR assays target only 9 to 24 STR sites, depending on the vendors. The accuracy of the PCR-based STR assay can be low in cases of close genetic relationships such as the authentication of murine cell lines derived from specific strains of inbred animals that lack unique genetic markers and different tumor cell lineages from the same human donor. Further, biosamples with mutations in mismatch repair (MMR) genes are known to exhibit microsatellite instability and a hypermutator phenotype. Consequently, this can lead to genetic drift and/or outgrowth of contaminating cells and STR misclassification.

With deep sequencing-based QC panel, you will achieve three-level sample authentication.

List of authenticatable human and mouse cell lines
NAR Genomics and Bioinformatics, Volume 2, Issue 3, September 2020, lqaa060