Microbiome Sequencing

Full-length 16s rRNA Sequencing

Crown Bioscience leads in comprehensive and cost-effective full-length 16S rRNA analysis using PacBio's long-read third-generation sequencing. Our approach offers distinct advantages over short read sequencing. By capturing the entire 16S rRNA gene, we provide unparalleled insights into bacterial diversity and ensure precise taxonomic classification. Overcoming limitations of short read methods, such as primer bias and reduced taxonomic resolution, our full-length sequencing delivers a comprehensive and unbiased view of the microbial community. This empowers the identification of potential drug targets and deepens understanding of their functional roles.

Fig. Commonly used DNA regions in short-read and long-read 16S rRNA sequencing

Shotgun Metagenomic Sequencing

Metagenomic sequencing provides a significant advantage over 16S rRNA sequencing by capturing comprehensive genomic information. This empowers higher taxonomic resolution, direct functional profiling, and the identification of novel or rare species. While it comes at a higher cost and requires specialized bioinformatics expertise, shotgun sequencing is an ideal choice for researchers looking to delve into a smaller number of samples with greater depth compared to amplicon sequencing. Embrace the power of metagenomic sequencing to unlock a wealth of valuable insights about microbial communities and their functional potential.

Metatranscriptomic Sequencing 

Metatranscriptomic sequencing studies the gene expression profiles of microbial communities, providing insights into their functional activities and responses to the environment. By analyzing RNA molecules, researchers can identify actively expressed genes, revealing the metabolic pathways and biological processes within the microbiome. This approach enhances our understanding of how microbes interact with their surroundings and contribute to ecosystem functions and host-associated processes.