Are your cell lines and biological models REALLY what you think they are? According to multiple studies, 1/5 to 1/3 of popular cell lines have been contaminated by intraspecies/interspecies cells, mycoplasma and/or viruses.
Ensure confidence in your models and reproducibility of high quality data by using our deep sequencing-based cell line authentication service. The panel encompasses over 600 SNPs and chromosome segments to accurately characterize mouse and human samples including cell lines, organoids, xenograft models, and patient tissues. We have also generated unique DNA fingerprints for the most commonly used oncology research models, covering over 1,200 human cancer and 30 mouse cell lines.
With deep sequencing-based QC panel, you will achieve three-level sample authentication.
"We have used STR assays for cell line authentication in the past, but found that this technique lacks the sensitivity and ability to detect mouse cell contamination. Unlike other services, the cell line authentication service from Crown Bioscience provides a high-level of sensitivity and accuracy, and the ability to detect interspecies, and other, contamination so we can continue our ground-breaking research."
Professor Hongchun Liu,
Shanghai Institute of Materia Medica, Chinese Academy of Sciences
Additional services available:
SNP is especially useful for cells:
7-11 business days
Human gender is inferred via 3 SNPs on the Y chromosome. Please note that the Y chromosome is lost in some cancer genomes, therefore a predicted result of ‘female’ may not always be accurate.
Our panel can classify three reference populations, Han Chinese (CHB), Nigeria Yoruba (YRI), and Utah residents with Northern and Western European ancestry (CEU). The race can be predicted when >70% of total SNPs support one population.
Greater than 90% similarity ratio is considered a match.