10x Genomics Visium Technology

Crown Bioscience partners with you to deliver cutting-edge spatial gene expression profiling using 10x Genomics Visium technology. This revolutionary platform provides unparalleled resolution to explore gene expression patterns directly within the context of tissue architecture.

Unlock Spatial Insights

10x Genomics Visium technology offers groundbreaking spatial gene expression profiling. This cutting-edge platform enables researchers to visualize and analyze gene expression directly within the tissue’s spatial context. By integrating spatial and molecular data, our services empower you to uncover critical biological insights that are impossible with traditional bulk or single-cell RNA sequencing methods. By combining histology and whole transcriptome profiling (using fresh frozen or FFPE), this technology allows you to spatially map gene expression within tissues, providing valuable insights into cellular function and interactions. Whether you’re investigating tumor microenvironments, tumor heterogeneity, developmental biology, or other complex systems, our solution provides the precision and clarity needed to advance your discoveries.

Features and Benefits of 10x Genomics Visium

Feature	Benefit
High-resolution spatial gene expression mapping	Gain a detailed understanding of gene expression patterns within the native tissue context.
Compatibility with a variety of tissue types	Analyze fresh frozen or FFPE (formalin-fixed paraffin-embedded) tissue samples with confidence.
Advanced visualization tools	Generate clear, intuitive spatial maps for better biological interpretation.
Integration of morphological and molecular data	Reveal critical spatial relationships in complex tissue architectures.
Comprehensive downstream data analysis	Receive actionable insights with clear visualizations and tailored reports.

Product specifications

Sequencing based whole transcriptome analysis in the morphological context, targeting 18,000 human and 20,000 mouse genes (Visium CytAssist probe-based assays)

Compatibility:

Visium CytAssist probe-based assays for human and mouse Fresh Frozen or FFPE blocks, pre-sectioned FFPE tissue on glass slides
Visium PolyA capture-based direct placement method for Fresh Frozen (variable species) Tissue Analysis Size & Resolution:
- Standard Visium Slides with capture areas of 6.5 x 6.5 mm (~ 5000 gene expression spots) or 11 x 11 mm (~ 14,000 gene expression spots), barcoded gene expression spots are 55 µm in diameter and 100 µm distance from center to center between spots, providing an average resolution of 1 to 10 cells per spot.
- Visium HD Slides with capture areas of 6.5 x 6.5 mm with a continuous lawn of oligonucleotides in a grid of 2 µm barcoded squares, no gaps between barcoded oligonucleotids providing a single-scale resolution

Partner with Us

At Crown Bioscience, we are dedicated to advancing your research through cutting-edge technologies like Visium Spatial Transcriptomics. Our expertise in spatial transcriptomics, combined with comprehensive data analysis and tailored support, helps you unlock meaningful, actionable insights directly from tissue samples. Whether you’re investigating tumor microenvironments, tissue heterogeneity, or complex biological systems, our solutions empower you to make groundbreaking discoveries with unparalleled precision and clarity. Connect with us today to elevate your spatial biology research.

FAQs

What is Spatial Transcriptomics?

Spatial transcriptomics is a revolutionary technique that enables researchers to measure and map gene expression within the spatial context of intact tissue sections. Unlike traditional bulk RNA sequencing, which averages gene expression across all cells in a sample, spatial transcriptomics preserves the architectural organization of tissues, providing critical insights into how gene activity varies across different regions.

How Does Spatial Transcriptomics Work?

Spatial transcriptomics works by capturing mRNA transcripts from tissue sections placed on specially designed slides embedded with spatially barcoded oligonucleotides. These barcodes retain the positional information of each transcript, allowing for precise mapping of gene expression back to specific tissue regions. Technologies like 10x Genomics Visium integrate histological imaging with whole transcriptome sequencing, enabling researchers to correlate gene expression data with tissue morphology for comprehensive spatial analysis.

Why is Spatial Transcriptomics a valuable tool to better understand complex cellular relationships?

Spatial transcriptomics provides an unbiased, high-throughput approach to analyze thousands of genes at once within the histological context. It enables not only for visualization but also for quantification and comparison of gene expression. This allows for a more comprehensive view of the transcriptomic landscape, uncovering novel biomarkers, cellular interactions, and biological pathways or visualize and compare compound effects within the spatial context of a tissue sample, that may be missed with traditional bulk sequencing approaches.

Therefore, spatial transcriptomics can be a key application in exploratory research for biomarker discovery and hypothesis generation as well as a valuable tool within the drug development process to facilitate the holistic view of compound effects.