Peritonitis Models

Key Benefits

• Effectively determine preclinical drug efficacy and model response to treatment.
• Perform mechanistic assessment of anti-inflammatory compounds via rapid and simple assessment of leukocyte recruitment and acute inflammatory response.
• Partner with our experienced team for expert consultation and custom model builds

Choose from a range of models recapitulating various types of acute inflammation:

• Monosodium urate (MSU) induced peritonitis model
• Zymosan-induced peritonitis model
• fMLP, LPS/sepsis, and thioglycollate-induced models coming soon.

Monosodium Urate-Induced “Gout” Peritonitis Model

• Perform simple and rapid evaluation of agent inflammasome-mediated acute inflammatory response.
• Effectively characterize inflammatory exudates including PMNs and IL-1β, with ample volumes collected from peritoneal cavities compared to smaller joint synovial fluid volumes.
• Peritonitis induced via 3mg MSU crystal injection i.p., with fluid lavaged from peritoneal cavities 4-6 hours later.

Zymosan-Induced Peritonitis Model

• Rapidly assess an agent’s ability to attenuate acute TLR-activated inflammation associated with peritonitis.
• Accurately investigate your compounds’ effect on various immune components including neutrophils, eosinophils, and macrophages.
• Peritonitis induced by i.p. injection of 0.5mg zymosan, a yeast cell wall component and TLR2 agonist, with fluid lavaged from peritoneal cavities 6-48 hours later dependent upon immune cell populations of interest.

Evaluate Major Study Endpoints

• Flow cytometry analysis for leukocytes of interest such as PMNs and macrophages.
• Cytokine levels measured by ELISA or MSD.
• Inflammatory gene expression of recruited leukocytes measured by qPCR.