Lei Zheng, Daniel X. He, Ruilin Sun*, Annie X. An, Jian Fei”, Henry Q.X. Li, Davy X. Ouyang
*Shanghai Model Organisms Center, Inc., 3577 Jinke Rd, Shanghai, 201203 China
Combinations of immune checkpoint inhibitors (ICIs, e.g. PD-1 and CTLA-4) are being clinically assessed to try and overcome the low response rate seen for single agent use. To allow preclinical ICI combination testing, immunocompetent models are needed which can be used with pairs of human specific ICIs.
We’ve previously reported on our HuGEMM platform of immune checkpoint target humanised mice. Models are available for a variety of single immune checkpoints including PD-1, PD-L1, CTLA-4, OX40 etc for assessing the corresponding human antibody.
This poster details the generation of double knock-in HuGEMM mouse models, featuring human PD-1/PD-L1 and PD-1/CTLA-4 pairs, model validation, and model use in preclinically evaluating combination checkpoint inhibitor regimens.