Bryan Miller, Yanrui Song, Jason Davies, Rajendra Kumari, Davy Xuesong Ouyang, Henry Qixiang Li, Yinfei Yin
The tumor microenvironment (TME) plays a pivotal role in immunotherapy response, and needs to be considered in preclinical studies. Orthotopic syngeneic models provide a more clinically-relevant TME than subcutaneous alternatives, including recapitulating immune and stromal component interactions with the tumor.
Using bioluminescent imaging (BLI) allows real-time monitoring of orthotopic syngeneic tumor development and progression, as well as treatment response. This poster details the development of bioluminescent syngeneic cell lines, and comparison of therapeutic response of orthotopic implantation vs subcutaneous models.