AACR-NCI-EORTC Poster B041: Imaging Orthotopic Syngeneic Models

Establishment of Orthotopic Syngeneic Models using Bioluminescent Imaging to Recapitulate the Tumor Microenvironment and Evaluate Immunotherapies

Bryan Miller, Yanrui Song, Jason Davies, Rajendra Kumari, Davy Xuesong Ouyang, Henry Qixiang Li, Yinfei Yin

The tumor microenvironment (TME) plays a pivotal role in immunotherapy response, and needs to be considered in preclinical studies. Orthotopic syngeneic models provide a more clinically-relevant TME than subcutaneous alternatives, including recapitulating immune and stromal component interactions with the tumor.

Using bioluminescent imaging (BLI) allows real-time monitoring of orthotopic syngeneic tumor development and progression, as well as treatment response. This poster details the development of bioluminescent syngeneic cell lines, and comparison of therapeutic response of orthotopic implantation vs subcutaneous models.

Read this Poster to Discover:

• That syngeneic orthotopic models offer a valuable platform for testing immunotherapies, with a more clinically relevant TME compared to a subcutaneous site
  
  
• How implantation site impacts treatment response – the H22 subcutaneous model responds to anti-mCTLA-4 antibody, however the orthotopic model is resistant to the same dosing regimen
  
  
• How the Pan02 model shows greater response to anti-mCTLA-4 treatment in the orthotopic than subcutaneous setting

Download the Poster Now!