HuGEMM™ PD-1/OX40 Double Knock-In Mouse Model

Evaluate combination biological therapies in vivo with humanized drug target mouse models

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The HuGEMM PD-1/OX40 mouse provides a translational humanized drug target mouse model for testing human-specific combination therapies. The double knock-in model has both humanized PD-1 and OX40 receptors within a functional mouse immune system.

The PD-1/OX40 double knock-in model was developed by breeding the PD-1 HuGEMM, a chimeric model generated via knock-in of human exon 2 and 3 of the PD-1 gene, and the OX40 HuGEMM, a model generated by replacing the entire mOX40 coding region with the human counterpart.

  • Evaluate your human-specific combinations of anti-PD-1/anti-OX40 therapies in vivo

  • Save study resources by using a cost effective alternative to more complex fully humanized models

Model Characterization Data

Human transgene expression was validated via FACS in the PD-1 (Figure 1) and OX40 single KI HuGEMM model.

Figure 1: Human PD-1 expression on T cells is confirmed by FACS analysis in the single KI HuGEMM model.

Figure 2: Validation of efficacy of human-specific anti-PD-1 and anti-OX40 antibodies: Combination anti-PD-1 + anti-OX40 yields a better response than single agent treatments, with 5/7 mice cured in the combo group