HuGEMM™ Human CD3E Transgenic Model

Humanized target model for evaluating the efficacy of CD3-based bispecific antibodies


Engineered Models for Human CD3-Targeted Immunotherapy Evaluation

HuGEMM CD3E mice provide translational humanized drug target mouse models for testing CD3E human-specific targeted agents. These transgenic models have a humanized CD3 (hCD3) co-receptor within a functional mouse immune system. This allows the evaluation of human-specific immunotherapies which recognize hCD3, such as CD3-targeted bispecific antibodies and BiTE® molecules.

Benefits of using HuGEMM CD3E mice:

  • Understand immune effects of your hCD3-specific molecule in the context of normal T cell development, unlike previously developed models
  • Elicit strong T cell activation after anti-CD3E stimulation, with consistent cytokine secretion
  • Robust evaluation of T cell redirecting antibodies targeting human CD3E in vivo in a fully immunocompetent environment
  • Avoid donor-to-donor variability and increase reproducibility of your bispecific antibody research by using mice T cells from a transgenic mouse model expressing hCD3, as an alternative to human PBMCs

Translational Platform Pairing for Customized CD3-Targeted Bispecific Antibody Assessment

Gain preclinical insights on the efficacy, potency, and selectivity of CD3-targeted bispecific T-cell engagers by combining the HuGEMM CD3E model with HuCELL™, which are engineered syngeneic cell lines expressing the human associated antigen (hTAA) of interest. CrownBio’s tailored CD3-targeted bispecific antibody solutions enable you to:

  • Assess the target engagement, potency, and immune effects in vitro by co-culturing hCD3 T cells with HuCELL
  • Evaluate the efficacy and safety in vivo in an immunocompetent setting by pairing CD3 HuGEMM with HuCELL

Human CD3E Model Bite Assessment

Human CD3E HuGEMM Model Information  

hTAA Expressing Syngeneic Lines (HuCELL) Validated in CD3E HuGEMM  

Discuss Your CD3 Targeted Bispecific Antibody Project