HuGEMM™ PD-1/CTLA-4 Double Knock-In Mouse Model

Evaluate combinations of checkpoint inhibitors in vivo with humanized drug target mouse models

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HuGEMM humanized PD-1/CTLA-4 mice provide a translational model for testing human-specific combination therapies. This double knock-in model has both humanized PD-1 and CTLA-4 receptors within a functional mouse immune system.

The PD-1/CTLA-4 double knock-in model was developed by breeding two single knock-in models, the PD-1 and CTLA-4 mice, which express either human PD-1 and CTLA-4 under the respective endogenous murine promoters. This supports the in vivo evaluation of human-specific combination therapies, blocking multiple immune checkpoints simultaneously.

  • Evaluate human-specific combinations of anti-PD-1/anti-CTLA-4 therapies in vivo

  • Save study resources by using a cost effective alternative to more complex fully humanized models

Model Characterization Data

Figure 1: Human PD-1 or human CTLA-4 expression on T cells is confirmed by FACS analysis in single KI HuGEMM models.

Figure 2: Validation of efficacy of human-specific anti-PD-1 and anti-CTLA-4 antibodies: Combination anti-PD-1 + anti-CTLA-4 results in complete remission. Cured mice remain disease free up to 40 days post grouping. Rechallenge of cured mice with tumor cells implanted in the left flank results in memory response with tumors failing to grow.

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