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AACR 2023 Poster 6775

Indivumed Services - Combination of Spatial Transcriptomics and Multiplex IHC as Valuable Multi-Omics Tool for Biomarker Discovery

Nicole Kerstedt, Monika Schoeppler, Bita Motamedi-Baniassad, Antonia Hirt, Julia Ploetzky, Mirja Kastein, Laura Thoms, Malik Khenkhar, Hartmut Juhl, and Kerstin A. David

Colorectal cancer (CRC) is one of the most common cancers worldwide and shows heterogenous molecular subtypes making personalized medicine as well as identifying new targets highly important to provide individual patients with the most appropriate treatment. To gain more insight into the visualization of potential molecules or pathways for targeting CRC we used a multi-omics approach for the simultaneous analysis of gene and protein expression within a sample. In this study we combined fluorescent multiplex immunohistochemistry (mIHC) for the detection of proteins (3-plex panel & DAPI) with the Visium Spatial Transcriptomics technology (10x Genomics) that enables the detection of the whole transcriptome in the histological context.

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  • Details on the study method used including tissue preparation, sample processing, imaging, library preparation, sequencing, and data processing.

  • Observed correlations of protein and gene expression of Ki-67, p53, and p21 within the CRC tumor sample.

  • How the combination of fluorescent mIHC and Spatial Transcriptomics can be used to enable the discovery of new biomarkers or pathways predominantly linked to CRC tissue that may serve as new targets for cancer treatment.

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