AACR 2023 Poster 52

Characterization of Murine Intrahepatic Cholangiocarcinoma Homograft Models for Therapeutic Evaluation

Jinxi Wang¹, Leilei Chen¹, Likun Zhang¹, Ruilin Sun², Jingqian Zhu¹, Lei Zheng¹, Ludovic Bourre¹, Jessie J.J. Wang<sup>1

1</sup>Crown Bioscience Inc., 16550 West Bernardo Drive, Building 5, Suite 525, San Diego, CA 92127
²Shanghai Model Organisms Center, Inc. (SMOC), 178 Banxia Road, Pudong New Area, Shanghai

Intrahepatic cholangiocarcinoma (iCCA) is a highly aggressive adenocarcinoma derived from bile ducts within the liver and establishing suitable animal models for in vivo investigation of oncology and immuno-oncology treatment strategies for iCCA is critical. In this study, genetically engineered mouse models (GEMMs) were established with liver-specific Kras^G12D/+ and Pten^null mutations and successfully induced development of iCCA liver lesions with glandular morphology in C57BL/6 mice. Although the incidence rate of iCCA in this GEMM reached almost 100%, there are some limitations. This can be circumvented by using tumor homograft models combining the benefits of GEMM with an improved operational simplicity, consistency and growth.

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• Strategy for generating GEMM with spontaneous iCCA and tumor homograft model
  
  
• Advantages of using tumor homograft models and combining the benefits of GEMM models
  
  
• Characterization data of  iCCA homograft models, derived from different iCCA GEMM individuals

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