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Next Generation Sequencing Services

We offer high-throughput sequencing using second and third generation sequencing and optical genome mapping platforms to deliver high-quality, interpretable results. 


RNA Sequencing (RNA-Seq)

 

Our RNA-Seq platforms are compatible with a variety of sample types, including cell lines, snap-frozen tissue, whole peripheral blood, FFPE blocks and sections, peripheral blood mononuclear cells (PBMCs), total RNA, and biopsy tissue. Compatible library preparation kits include non-Illumina for mRNA, Illumina Truseq for mRNA, and ribosomal RNA-seq for mRNA, lncRNA, and microRNA. 

Applications: 

  • Differential gene expression 
  • Functional annotation, pathway and network analysis 
  • Driver mutation prediction 
  • Tumor purity analysis 
  • Molecular subtyping analysis 
  • Comparative analysis with published human tumor samples upon request 
  • Options to accommodate other analyse

Whole Exome Sequencing (WES)

 

Our WES service is suitable for sequencing-based analysis of cell lines, snap-frozen tissue, whole peripheral blood, FFPE blocks and sections, PBMCs, biopsy tissue, and mouth swabs. 

Applications: 

  • Identify genomic variants on DNA level, such as gene mutation, and copy number variants 
  • Co-expression network analysis 
  • Comparative analysis with published human tumor samples upon request 
  • Options to accommodate other analyses 

Whole Genome Sequencing (WGS) / Whole Genome Bisulfite Sequencing (WGBS)

 

WGS/WGBS platforms are compatible with multiple sample types, including cell lines, snap-frozen tissue, whole peripheral bloodFFPE blocks and sections, PBMCs, biopsy tissue, and mouth swabs. 

WGS applications: 

  • Gene mutation identification 
  • Copy number variants 
  • Large structural variants 

WGBS applications: 

  • Epigenomics 
  • Gene methylation analysis

Single Cell Sequencing (10x Genomics)

 

Our single cell sequencing service is suitable for analysis of cell lines, fresh tissue, whole peripheral blood, and PBMCs in single cell RNA-Seq and T cell receptor (TCR) sequencing assays 

Applications: 

  • Characterize and identify heterogeneous cell populations 
  • Discover new cell markers and regulatory pathways 
  • Uncover novel cell types, cell states & rare cell types 
  • Reconstruct developmental hierarchies and reveal lineage relationships 
  • Profiling healthy and diseased tissue and organs 

We also offer bioinformatics analysis for our single cell sequencing service, which includes: 

  • QC and cell/gene filtering 
  • Cluster analysis on integrated samples 
  • Exploration of known cell type markers 
  • Identification of cluster-specific markers 
  • Functional analysis on cluster-specific markers 
  • Further customized analysis upon request 

single-sequencing-value

 

Third Generation Sequencing – PacBio Sequel II Platform

 

Crown Bioscience uses the PacBio Sequel II platform, which offers single-molecule, real-time (SMRT) sequencing. Long reads are readily assembled into complete genomes for sequencing of full-length transcripts with >99.999% consensus accuracy. Other benefits include uniform coverage of typically inaccessible regions, single-molecule resolution, and direct detection of epigenetic modifications. 

Applications: 

  • WGS—de novo assembly, variant detection, and structural variant detection 
  • RNA sequencing—full-length transcripts for whole transcriptome and genome annotation 
  • Complex populations—full-length 16S, metagenomic functional profiling, and shotgun metagenomic assembly 

Optical Genome Mapping – Bionano Saphyr® Platform

 

The Bionano Saphyr platform resolves large-scale structural variations including homozygous insertions/deletions, balanced and unbalanced translocations, inversions, duplications, and copy number variations with >95% sensitivity and a false positive rate of less than 2%. 

Applications: 

  • Undiagnosed genetic disorders 
  • Solid tumor research 
  • Cell line stability 

Microbiome Analysis  

 

The PacBio Sequel II and Illumina Novaseq 6000 platforms for microbiome culture and analysis are compatible with feces, DNA, tissue and microbiome samples.  

Applications: 

  • Full-length 16S rRNA sequencing with PacBio Sequel II platform) 
  • Metagenomic sequencing
  • Metatranscriptomic sequencing


 

Nanostring Services

 

We utilize the nCounter® platform for Nanostring analysis.  

  • Array-based multiplex analysis of up to 800 RNA, DNA, or protein targets
  • Flexible sample types including FFPE, tissue, lysates, and biofluid samples
  • Panels available:
  • PanCancer IO 360 Panel
  • PanCancer Immune Profiling Panel
  • PanCancer Pathways Panel
  • PanCancer Progression Panel
  • Check other available panels here

Applications : 

  • Gene expression analysis 
  • Solid tumor profiling 
  • Immuno-oncology profiling 
  • Gene fusion analysis 
  • Single cell gene expression analysis 
  • miRNA expression analysis 
  • Copy number variation analysis 
  • lncRNA expression analysis 
  • ChIP-String expression analysis 

Ready to get started?

Reach out to our experts for more information about our genomics services or to request support with your genomics research. 

Learn More?

Download the Factsheet to learn more our comprehensive genomics and high-throughput sequencing services.