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Cell Line and Model Authentication

Ensure the reproducibility of your research with our barcode deep NGS-based oncology cell line and model authentication

banner-oncology-model-authentication

Highly Sensitive Authentication Service for your Oncology Samples

Ensure confidence in your models and reproducibility of high quality data by using our NGS-based cell line and model authentication service. The panel encompasses over 600 SNPs and chromosome segments to accurately characterize mouse and human samples including cell lines, organoids, xenograft models, and patient tissues. We have also generated unique DNA fingerprints for the most commonly used oncology research models, covering over 1,200 human cancer and 30 syngeneic cell lines.

Our cell line and model authentication service enables you to:

  • Confidently authenticate mouse and human cell lines and models for increased reliability and reproducibility, and decreased model misidentification and contamination
  • Routinely monitor newly developed models by establishing unique DNA fingerprints
  • Easily understand your results via processed data and a comprehensive summary report, and support through consultation as needed
  • Generate high quality, reliable data for grant applications, publications, and regulatory submissions
Basic and Comprehensive Service Features

Determine oncology cell line and model identity with CrownBio Cell Line and Model AuthenticationDetermine the identity of your mouse and human cell lines and models

Identify potentially misidentified oncology cell lines with CrownBio Cell Line and Model AuthenticationIdentify potentially misidentified cell lines

Compare samples similarity with CrownBio Cell Line and Model AuthenticationQuantify the match percentage in comparison to reference profiles and/or another submitted specimen

Comprehensive Service: Additional Included Features

Ensure oncology experiment reproducibility with CrownBio Cell Line and Model AuthenticationEnsure the integrity and reproducibility of your oncology experiments

Detect model contamination with CrownBio Cell Line and Model AuthenticationDetect intra- and mouse-human interspecies contamination with 1-2% sensitivity

Remove cross-contamination of oncology cell lines with CrownBio Cell Line and Model AuthenticationRule out the possibility of cross-contamination of your mouse and human cell lines

Detect murine stroma in xenograft tumors with CrownBio Cell Line and Model AuthenticationDetect mouse stromal invasion of xenograft tumors

Determine contamination sources with CrownBio Cell Line and Model AuthenticationGain insight into the root cause of sample contamination

Perform mycoplasma testing with CrownBio Cell Line and Model AuthenticationCheck for mycoplasma and viral contamination that pose risks for your research and lab safety

Annotate you oncology human and preclinical samples with CrownBio Cell Line and Model AuthenticationAnnotate your human preclinical and RUO samples with gender and race information

Choose from Two Model Authentication Options,
to Best Fit your Quality Control Needs

Deliverables
 
BASIC service
comprehensive service
Mouse
Human
Mouse
Human
Cell line and model identity
Reference profile for comparison (if applicable)
Percent match calculation vs. reference profile and/or specimen (if applicable)
Intraspecies contamination
 
 
Mouse-human interspecies contamination
 
 
 
Determination of contaminant identity for those with established SNP fingerprints and reference profiles on CCLE
 
 

Estimation of contamination ratio
 
 
Mycoplasma test (all mycoplasma species and 11 mollicutes)
 
 
Viral status (includes CMV, HIV, HPV16, HPV18, EBV)
 
 
Human gender determination
 
 
 
Human race determination
 
 
 
Nucleic acid extraction
Processed data
Summary report
Consultation before and after testing

Sample Requirements and Submission  

Frequently Asked Questions