Cell Line and Model Authentication

Highly Sensitive Authentication Service for your Oncology Samples

Ensure confidence in your models and reproducibility of high quality data by using our NGS-based cell line and model authentication service. The panel encompasses over 600 SNPs and chromosome segments to accurately characterize mouse and human samples including cell lines, organoids, xenograft models, and patient tissues. We have also generated unique DNA fingerprints for the most commonly used oncology research models, covering over 1,200 human cancer and 30 syngeneic cell lines.

Our cell line and model authentication service enables you to:

Confidently authenticate mouse and human cell lines and models for increased reliability and reproducibility, and decreased model misidentification and contamination

Routinely monitor newly developed models by establishing unique DNA fingerprints

Easily understand your results via processed data and a comprehensive summary report, and support through consultation as needed

Generate high quality, reliable data for grant applications, publications, and regulatory submissions

Basic and Comprehensive Service Features
Determine the identity of your mouse and human cell lines and models Identify potentially misidentified cell lines Quantify the match percentage in comparison to reference profiles and/or another submitted specimen

Basic and Comprehensive Service Features

Determine oncology cell line and model identity with CrownBio Cell Line and Model Authentication Determine the identity of your mouse and human cell lines and models

Identify potentially misidentified cell lines

Compare samples similarity with CrownBio Cell Line and Model Authentication Quantify the match percentage in comparison to reference profiles and/or another submitted specimen

Comprehensive Service: Additional Included Features
Ensure the integrity and reproducibility of your oncology experiments Detect intra- and mouse-human interspecies contamination with 1-2% sensitivity Rule out the possibility of cross-contamination of your mouse and human cell lines Detect mouse stromal invasion of xenograft tumors	Gain insight into the root cause of sample contamination Check for mycoplasma and viral contamination that pose risks for your research and lab safety Annotate your human preclinical and RUO samples with gender and race information

Comprehensive Service: Additional Included Features

Ensure oncology experiment reproducibility with CrownBio Cell Line and Model Authentication Ensure the integrity and reproducibility of your oncology experiments

Detect model contamination with CrownBio Cell Line and Model Authentication Detect intra- and mouse-human interspecies contamination with 1-2% sensitivity

Remove cross-contamination of oncology cell lines with CrownBio Cell Line and Model Authentication Rule out the possibility of cross-contamination of your mouse and human cell lines

Detect murine stroma in xenograft tumors with CrownBio Cell Line and Model Authentication Detect mouse stromal invasion of xenograft tumors

Determine contamination sources with CrownBio Cell Line and Model Authentication Gain insight into the root cause of sample contamination

Perform mycoplasma testing with CrownBio Cell Line and Model Authentication Check for mycoplasma and viral contamination that pose risks for your research and lab safety

Annotate you oncology human and preclinical samples with CrownBio Cell Line and Model Authentication Annotate your human preclinical and RUO samples with gender and race information

Choose from Two Model Authentication Options,
to Best Fit your Quality Control Needs

Deliverables

BASIC service

comprehensive service

Mouse

Human

Mouse

Human

Cell line and model identity

Reference profile for comparison (if applicable)

Percent match calculation vs. reference profile and/or specimen (if applicable)

Intraspecies contamination

Mouse-human interspecies contamination

Determination of contaminant identity for those with established SNP fingerprints and reference profiles on CCLE

Estimation of contamination ratio

Mycoplasma test (all mycoplasma species and 11 mollicutes)

Viral status (includes CMV, HIV, HPV16, HPV18, EBV)

Human gender determination

Human race determination

Nucleic acid extraction

Processed data

Summary report

Consultation before and after testing

Sample Type		Amount	Shipping Conditions
DNA	Powder	100-500ug	Room tempature (RT)
DNA	Liquid	100-500ug	Wet ice
Cell Pellet		At least 1 million cells	Dry ice
Fresh Tumor Tissue	Snap Frozen	At least 200mg or 1 million cells	Dry ice
Fresh Tumor Tissue	Formalin Fixed	At least 200mg or 1 million cells	RT for delivery within 72hr
FFPE Tissue		Unstained tissue sections (and H&E stained adjacent sections)	RT