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NGS-based SNP Profiling for Cell Line and Model Authentication

Ensure the reproducibility of your research with our Next-Generation-Sequencing-based SNP genotyping service

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Are your cell lines and biological models REALLY what you think they are? According to multiple studies, one fifth to one third of popular cell lines have been contaminated by intraspecies/interspecies cell lines, mycoplasma and/or viruses.

Ensure confidence in your models and reproducibility of high quality data by using our NGS-based SNP profiling cell line and model authentication service. The panel encompasses over 600 SNPs and chromosome segments to accurately characterize mouse and human samples including cell lines, organoids, xenograft models, and patient tissues. We have also generated unique DNA fingerprints for the most commonly used oncology research models, covering over 1,200 human cancer and 30 syngeneic cell lines.

NGS-based SNP Profiling for Cell Line and Model Authentication

With NGS-based SNP profiling, you will achieve three-level sample authentication.

Client Testimonial

"We have used STR assays for cell line authentication in the past, but found that this technique lacks the sensitivity and ability to detect mouse cell contamination. Unlike other services, the cell line authentication service from CrownBio provides a high-level of sensitivity and accuracy, and the ability to detect interspecies, and other, contamination so we can continue our ground-breaking research."

Professor Hongchun Liu,
Shanghai Institute of Materia Medica, Chinese Academy of Sciences

Assay Comparison NGS-based SNP Profiling STR Profiling SNP Profiling
Technology Barcode deep NGS Multiplex PCR & capillary electrophoresis Multiplex PCR/qPCR
Readout Type Digital (clean, near-zero quantification error) Analog (noisy, high quantification error) Analog (noisy, high quantification error)
Human Sample Authentication Yes Yes Yes
Mouse Sample Authentication Yes Limited No
MMR Deficient Cell lines identification Yes No Yes
Contamination-Detecting Sensitivity High (1%) Low to medium (5-20%) Low to medium (3-20%)
Accuracy High Low to medium Low to medium
Throughput High Low Low
Contaminant Identification Yes No No
Qualification of Contamination Ratio Yes No No
Suitable for Large Biobanks Yes No No
Interspecies Contamination Detection Yes Limited Limited
Intraspecies Contamination Detection Yes Limited Limited
Detecting Contamination w/o Reference Yes No No
Estimating Mix Ratios for 3+ Cell Lines Yes (1% sensitivity) No No

Additional services available:

  • Mycoplasma contamination check
  • Viral infection check
  • Gender and ethnicity identification for human samples
  • Genetic drift and constructing phylogeny of samples

List of authenticatable human and mouse cell lines at https://qc.crownbio.com
NAR Genomics and Bioinformatics, Volume 2, Issue 3, September 2020, lqaa060, https://doi.org/10.1093/nargab/lqaa060

Sample Requirements and Submission  

Frequently Asked Questions  



"This service provides a
high-level of sensitivity and accuracy, and the ability to detect interspecies contamination, so we can continue our ground-breaking research."

Professor Hongchun Liu,
CrownBio Client


Authenticate your Samples