|Sample Type||Amount||Shipping Conditions|
|DNA||Powder||100-500ug||Room tempature (RT)|
|Cell Pellet||At least 1 million cells||
|Fresh Tumor Tissue||Snap Frozen||At least 200mg or 1 million cells||Dry ice|
|Formalin Fixed||RT for delivery within 72hr|
|FFPE Tissue||Unstained tissue sections (and H&E stained adjacent sections)||RT|
Are your cell lines and biological models REALLY what you think they are? According to multiple studies, one fifth to one third of popular cell lines have been contaminated by intraspecies/interspecies cell lines, mycoplasma and/or viruses.
Ensure confidence in your models and reproducibility of high quality data by using our NGS-based SNP profiling cell line and model authentication service. The panel encompasses over 600 SNPs and chromosome segments to accurately characterize mouse and human samples including cell lines, organoids, xenograft models, and patient tissues. We have also generated unique DNA fingerprints for the most commonly used oncology research models, covering over 1,200 human cancer and 30 syngeneic cell lines.
NGS-based SNP Profiling for Cell Line and Model Authentication
With NGS-based SNP profiling, you will achieve three-level sample authentication.
"We have used STR assays for cell line authentication in the past, but found that this technique lacks the sensitivity and ability to detect mouse cell contamination. Unlike other services, the cell line authentication service from CrownBio provides a high-level of sensitivity and accuracy, and the ability to detect interspecies, and other, contamination so we can continue our ground-breaking research."
Professor Hongchun Liu,
Shanghai Institute of Materia Medica, Chinese Academy of Sciences
|Assay Comparison||NGS-based SNP Profiling||STR Profiling||SNP Profiling|
|Technology||Barcode deep NGS||Multiplex PCR & capillary electrophoresis||Multiplex PCR/qPCR|
|Readout Type||Digital (clean, near-zero quantification error)||Analog (noisy, high quantification error)||Analog (noisy, high quantification error)|
|Human Sample Authentication||Yes||Yes||Yes|
|Mouse Sample Authentication||Yes||Limited||No|
|MMR Deficient Cell lines identification||Yes||No||Yes|
|Contamination-Detecting Sensitivity||High (1%)||Low to medium (5-20%)||Low to medium (3-20%)|
|Accuracy||High||Low to medium||Low to medium|
|Qualification of Contamination Ratio||Yes||No||No|
|Suitable for Large Biobanks||Yes||No||No|
|Interspecies Contamination Detection||Yes||Limited||Limited|
|Intraspecies Contamination Detection||Yes||Limited||Limited|
|Detecting Contamination w/o Reference||Yes||No||No|
|Estimating Mix Ratios for 3+ Cell Lines||Yes (1% sensitivity)||No||No|
Additional services available:
- Mycoplasma contamination check
- Viral infection check
- Gender and ethnicity identification for human samples
- Genetic drift and constructing phylogeny of samples
Sample Requirements and Submission
Frequently Asked Questions
What types of cells and models are better characterized with SNP compared to STR?
SNP is especially useful for cells:
- With deficiencies in mismatch repair/microsatellite instability
- Derived from closely related inbred strains
- With chromosomal copy number aberrations
What is the turnaround time?
7-11 business days
How is gender determined?
Human gender is inferred via 3 SNPs on the Y chromosome. Please note that the Y chromosome is lost in some cancer genomes, therefore a predicted result of ‘female’ may not always be accurate.
Which human races can be determined?
Our panel can classify three reference populations, Han Chinese (CHB), Nigeria Yoruba (YRI), and Utah residents with Northern and Western European ancestry (CEU). The race can be predicted when >70% of total SNPs support one population.
What is considered a ‘match’?
Greater than 90% similarity ratio is considered a match.