Conventional and Unique, Proprietary T2D Models
In vivo measurement of glucose and insulin levels are the gold standard for testing your antidiabetic compound efficacy. At CrownBio we can assess the level of circulating sugar or insulin in rodent studies via:
- Single point measurements via glucometer
- Continuous telemetry monitoring
- Glucose and insulin tolerance tests
- ELISA based assays for insulin determination
- Glucose clamp tests to quantify insulin resistance.
At CrownBio we understand the need to maintain consistency with historical study data, therefore we can run your study with any commercially available rat or mouse model including rodent models of Type 2 diabetes (T2D) with monogenic mutations:
- ZDF and ZSF-1 rats
- db/db and ob/ob mice
Our CardioVascular and Metabolic Disease (CVMD) Translational Technology Platform also provides our highly translatable, proprietary models of T2D:
The MS-NASH (formerly called FATZO) mouse and ZDSD rat are polygenic models of dysmetabolism, spontaneously developing metabolic syndrome, obesity, and diabetes, which more closely mimic the human condition than models with leptin/leptin receptor mutations. Both models respond to antidiabetic treatments, providing highly translatable models for efficacy evaluations.
The MS-NASH Mouse and ZDSD Rat vs Conventional Type 2 Diabetes Models
|Genetics of the disease||Polygenic||Polygenic, diet-induced||Monogenic, relies on leptin mutation||Monogenic, relies on leptin mutation|
|Obesity/adiposity||Moderate (30%)||Moderate (30%)||Severe (>40%)||Moderate (>30%)|
|Insulin resistance||Rapid||Rapid, diet-induced||Rapid||Rapid|
|Hyperlipidemia||Moderate||Minimal||HDL only||HDL only|
|Leptin pathway||Intact||Leprfa, defective||Leprfa, defective||Intact|
|Hyperglycemia/overt diabetes||Yes, from 19 weeks||Yes, from 8-11 weeks||No||No|
|Beta cell failure||Late||Early||No||No|
Male MS-NASH mice become hyperglycemic at 14 weeks of age, and also develop insulin resistance at an early age.
The ZDSD rat progresses through T2D similarly to humans, with a bona fide prediabetic state occurring at 8-16 weeks of age. The model develops overt diabetes from 16 weeks of age and diabetic complications from 24 weeks of age.
Industry Standard Models of T1D
We also provide models of Type 1 diabetes such as:
- Streptozotocin (STZ) induced diabetic rat and mouse models
- Spontaneously occurring disease models: NOD mice, the BB rat, NRG-Akita mouse with immune deficiency
At CrownBio we also routinely run a variety of in vitro assays including the evaluation of glucose stimulated insulin secretion on isolated primary islets from various rodent models.