AACR 2022 Poster 2059

Immunotherapy Testing in 3D Ex Vivo Patient Tissue Platform with Preserved Tumor Microenvironment

Nataliia Beztsinna¹, Niels Meesters¹, Fanny Grillet², Donny van der Meer², Lidia Daszkiewicz¹, Kuan Yan¹, Emma Spanjaard¹, Willemijn Vader² and Leo Price^1
1 Crown Bioscience Netherlands, Leiden, The Netherlands, ² VitroScan B.V. Leiden, The Netherlands

Extensive applications of immunotherapies to treat cancer patients has been hampered by low response rates in the clinic (about 20%)¹ and high failure rates of clinical trials (about 90%)². One of the underlying reasons is a lack of translational preclinical models that accurately replicate human immunological complexity. Even the most advanced 3D in vitro models, such as patient-derived organoids, are still lacking the innate immune components of the tumor microenvironment (TME).

This poster presents a novel 3D Ex Vivo Patient Tissue Platform that combines a short-term 3D ex vivo tumor culture system with high content image (HCI)-based analysis.

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• Successfully combined immunotherapy testing protocols using fresh patient tumor tissue with preserved TME components and advanced 3D HCI analysis.
  
  
• Rapid, reliable and patient-relevant approach to test immunotherapies, including checkpoint inhibitors, for different solid tumor types.
  
  
• The potential to significantly improve the preclinical evaluation of immunotherapies and support the decision-making process during progression of drug candidates to the clinic.

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