SITC 2023 Poster 963

TME Immune-profiling of Ex Vivo Tumor Tissues and Their Functional Responses to Immuno-oncology Treatments

Sander Basten, Talita Stessuk, Ezgi Kaya Aksoy, Niels Meesters, Kuan Yan, Emma Spanjaard, Leo Price, Nataliia Beztsinna

Crown Bioscience, Leiden, The Netherlands

This research describes a study aimed at demonstrating the ability to measure tumor cell killing including checkpoint inhibition in the 3D Ex Vivo Patient Tissue platform using HCI in patient samples from multiple cancer indications.

Extensive biomarker characterization and extended flow panels were analysed to provide more granular immune profiling.

The 3D Ex Vivo Patient Tissue platform combines short-term 3D ex vivo tumor culture systems from fresh human material with high content imaging analysis. The platform preserves the innate tumor microenvironment, including resident immune cells, and thus allows immunological testing in ‘close-to-patient’ scenario.

Download this Poster to Discover:

• Ex vivo Patient Tissue Platform workflow and TME (tumor microenvironment) HCI workflow
  
  
• Heatmap representing ex vivo responses of NSCLC patient tissues to immuno-oncology treatments
  
  
• ICI response profiling in NSCLC resections using functional HCI assay. Sensitivity to superantigen (SEA) stimulation and/or treatment with CD3/CD28 beads and to clinically approved ICI.
  
  
• Flow cytometry immune-profiling of tumor samples

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