AACR 2026 Poster 1530

Comprehensive Preclinical Evaluation of CAR-T Cell Therapeutics in Organoid-Fibroblast Co-cultures

Yijie Ren, Tomas Veenendaal, Puxin Wang, Jiawen Gao, Jiawei Meng, Danting Yan, Yingying Li, Meng Zhang, Michelle Kop, Ashgard Weterings, Marten Hornsveld, Gera Goverse, Ludovic Bourre, Peng Wang, Jun Zhou

Discover how advanced co-culture models and high-content imaging enable deeper insights into CAR-T efficacy within complex tumor microenvironments.

Traditional 2D and simple 3D assays fail to fully capture the complexity of tumor immune interactions. This study demonstrates how organoid fibroblast co-culture systems combined with high-content imaging (HCI) provide a more physiologically relevant platform to evaluate CAR-T therapies. By incorporating stromal components and patient derived models, this approach enables improved assessment of tumor killing, immune infiltration, and resistance mechanisms, bridging the gap between mechanistic insights and translational relevance in immuno-oncology.

Download this Poster to Discover:

• Compare CAR-T performance across model complexity: Evaluate differences in CAR-T efficacy between PBMC-only, organoid co-culture, and fibroblast containing systems.
  
  
• Assess target-specific CAR-T activity: Visualize immune-tumor interactions in 3D: Leverage high-content imaging to quantify tumor volume, CAR-T infiltration, and cytotoxic effects in real time.
  
  
• Understand the role of the tumor microenvironment (TME): Explore how fibroblast-rich environments suppress CAR-T activity and reveal mechanisms of resistance.
  
  
• Select the right translational model: Learn how co-culture complexity impacts biological relevance, scalability, and predictive power in preclinical IO studies.

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