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In Vitro

Boost oncology drug discovery with XenoBase®, featuring the largest cell line selection and exclusive 3D organoid models. Benefit from OrganoidXplore™ and OmniScreen™ for rapid, in-depth analysis.

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In Vivo

Enhance drug development with our validated in vivo models, in vitro/ex vivo assays, and in silico modeling. Tailored solutions to optimize your candidates.

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Tissue

Experience ISO-certified biobanking quality. Access top biospecimens from a global clinical network, annotated by experts for precise research.

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Biomarkers and Bioanalysis

Leverage our global labs and 150+ scientists for fast, tailored project execution. Benefit from our expertise, cutting-edge tech, and validated workflows for reliable data outcomes.

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Data Science and Bioinformatics

Harness your data and discover biomarkers with our top bioinformatics expertise. Maximize data value and gain critical insights to accelerate drug discovery and elevate projects.

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KRAS

Accelerate innovative cancer treatments with our advanced models and precise drug screening for KRAS mutations, efficiently turning insights into clinical breakthroughs.

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EGFR

Advance translational pharmacology with our diverse pre-clinical models, robust assays, and data science-driven biomarker analysis, multi-omics, and spatial biology.

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Drug Resistance

Our suite integrates preclinical solutions, bioanalytical read-outs, and multi-omics to uncover drug resistance markers and expedite discovery with our unique four-step strategy.

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Patient Tissue

Enhance treatments with our human tumor and mouse models, including xenografts and organoids, for accurate cancer biology representation.

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Bioinformatics

Apply the most appropriate in silico framework to your pharmacology data or historical datasets to elevate your study design and analysis, and to improve your chances of clinical success.

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Biomarker Analysis

Integrate advanced statistics into your drug development projects to gain significant biological insight into your therapeutic candidate, with our expert team of bioinformaticians.

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CRISPR/Cas9

Accelerate your discoveries with our reliable CRISPR solutions. Our global CRISPR licenses cover an integrated drug discovery platform for in vitro and in vivo efficacy studies.

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Genomics

Rely on our experienced genomics services to deliver high quality, interpretable results using highly sensitive PCR-based, real-time PCR, and NGS technologies and advanced data analytics.

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In Vitro High Content Imaging

Gain more insights into tumor growth and disease progression by leveraging our 2D and 3D fluorescence optical imaging.

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Mass Spectrometry-based Proteomics

Next-generation ion mobility mass spectrometry (MS)-based proteomics services available globally to help meet your study needs.

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Ex Vivo Patient Tissue

Gain better insight into the phenotypic response of your therapeutic candidate in organoids and ex vivo patient tissue.

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Spatial Multi-Omics Analysis

Certified CRO services with NanoString GeoMx Digital Spatial Profiling.

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Biomarker Discovery

De-risk your drug development with early identification of candidate biomarkers and utilize our biomarker discovery services to optimize clinical trial design.

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DMPK Services

Rapidly evaluate your molecule’s pharmaceutical and safety properties with our in vivo drug metabolism and pharmacokinetic (DMPK) services to select the most robust drug formulations.

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Efficacy Testing

Explore how the novel HuGEMM™ and HuCELL™ platforms can assess the efficacy of your molecule and accelerate your immuno-oncology drug discovery programs.

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Laboratory Services

Employ cutting-edge multi-omics methods to obtain accurate and comprehensive data for optimal data-based decisions.

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Pharmacology & Bioanalytical Services

Leverage our suite of structural biology services including, recombinant protein expression and protein crystallography, and target validation services including RNAi.

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Screens

Find the most appropriate screen to accelerate your drug development: discover in vivo screens with MuScreen™ and in vitro cell line screening with OmniScreen™.

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Toxicology

Carry out safety pharmacology studies as standalone assessments or embedded within our overall toxicological profiling to assess cardiovascular, metabolic and renal/urinary systems.

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Our Company

Global CRO in California, USA offering preclinical and translational oncology platforms with high-quality in vivo, in vitro, and ex vivo models.

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Our Purpose

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We build a sustainable future by supporting employee growth, fostering leadership, and exceeding customer needs. Our values focus on innovation, social responsibility, and community well-being.

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Meet Our Leadership Team

We build a sustainable future by fostering leadership, employee growth, and exceeding customer needs with innovation and social responsibility.

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Scientific Advisory Board

Our Scientific Advisory Board of experts shapes our strategy and ensures top scientific standards in research and development.

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Application Note: HCC827 NSCLC Cell Line Derived Xenograft Model

Evaluate immunotherapeutics and agents to overcome drug resistance with our NSCLC EGFR exon 19 deletion resources

CrownBio Application Note: HCC827 NSCLC Cell Line Derived Xenograft Model

The HCC827 Non Small Cell Lung Cancer Cell Line (NSCLC) Derived Xenograft model contains an adenocarcinoma cell line which harbors an activating EGFR mutation in exon 19. This mutation, which occurs in approximately 48% of all EGFR-mutated NSCLC cases, confers increased sensitivity to EGFR tyrosine Kinase Inhibitors (TKIs), which has been validated in HCC827. This model also exhibits high expression of PD-L1, which can lead to cancer immune escape. These features of the HCC827 makes it a relevant model for NSCLC preclinical studies.

Positive clinical responses to EGFR mutated NSCLC cases using EGFR TKIs are observed, but after 10-16 months of treatment, drug resistance to EGFR TKIs commonly occurs, resulting in patient relapse. Crown Bioscience generated drug-resistant models of HCC827 via continuous exposure to specific EGFR TKIs and identified the mechanism of resistance in these models. These models have been subsequently used in preclinical development studies of new treatments for drug-resistant NSCLC cases.

Lack of immunotherapy models is a major challenge in developing improved immunotherapeutic treatments. The high expression of PD-L1 in model HCC827 makes it a suitable model for immunotherapeutic studies. By using the Crown Bioscience MiXenoTM platform, a HCC827 model with transient human immunity was generated to allow for evaluation of anti-PD-1 and anti-PD-L1 checkpoint inhibitor treatment.

Download This Application Note to Learn About:

  • The HCC827 validated cell line derived xenograft model, an adenocarcinoma cell line that contains patient-relevant activating EGFR mutation in exon 19
  • Generation of drug Resistant HCC827 NSCLC CDX models for understanding mechanisms of resistance and testing new targeted treatments
  • The HCC827 MiXenoTM model of transient human immunity and its utilization in the efficient evaluation of anti-PD-1 and anti-PD-L1 checkpoint inhibitors

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