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In Vitro

Boost oncology drug discovery with XenoBase®, featuring the largest cell line selection and exclusive 3D organoid models. Benefit from OrganoidXplore™ and OmniScreen™ for rapid, in-depth analysis.

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In Vivo

Enhance drug development with our validated in vivo models, in vitro/ex vivo assays, and in silico modeling. Tailored solutions to optimize your candidates.

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Tissue

Experience ISO-certified biobanking quality. Access top biospecimens from a global clinical network, annotated by experts for precise research.

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Biomarkers and Bioanalysis

Leverage our global labs and 150+ scientists for fast, tailored project execution. Benefit from our expertise, cutting-edge tech, and validated workflows for reliable data outcomes.

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Harness your data and discover biomarkers with our top bioinformatics expertise. Maximize data value and gain critical insights to accelerate drug discovery and elevate projects.

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KRAS

Accelerate innovative cancer treatments with our advanced models and precise drug screening for KRAS mutations, efficiently turning insights into clinical breakthroughs.

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EGFR

Advance translational pharmacology with our diverse pre-clinical models, robust assays, and data science-driven biomarker analysis, multi-omics, and spatial biology.

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Drug Resistance

Our suite integrates preclinical solutions, bioanalytical read-outs, and multi-omics to uncover drug resistance markers and expedite discovery with our unique four-step strategy.

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Patient Tissue

Enhance treatments with our human tumor and mouse models, including xenografts and organoids, for accurate cancer biology representation.

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Apply the most appropriate in silico framework to your pharmacology data or historical datasets to elevate your study design and analysis, and to improve your chances of clinical success.

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Integrate advanced statistics into your drug development projects to gain significant biological insight into your therapeutic candidate, with our expert team of bioinformaticians.

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Accelerate your discoveries with our reliable CRISPR solutions. Our global CRISPR licenses cover an integrated drug discovery platform for in vitro and in vivo efficacy studies.

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In Vitro High Content Imaging

Gain more insights into tumor growth and disease progression by leveraging our 2D and 3D fluorescence optical imaging.

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Mass Spectrometry-based Proteomics

Next-generation ion mobility mass spectrometry (MS)-based proteomics services available globally to help meet your study needs.

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Ex Vivo Patient Tissue

Gain better insight into the phenotypic response of your therapeutic candidate in organoids and ex vivo patient tissue.

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Spatial Multi-Omics Analysis

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De-risk your drug development with early identification of candidate biomarkers and utilize our biomarker discovery services to optimize clinical trial design.

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Rapidly evaluate your molecule’s pharmaceutical and safety properties with our in vivo drug metabolism and pharmacokinetic (DMPK) services to select the most robust drug formulations.

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Explore how the novel HuGEMM™ and HuCELL™ platforms can assess the efficacy of your molecule and accelerate your immuno-oncology drug discovery programs.

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Leverage our suite of structural biology services including, recombinant protein expression and protein crystallography, and target validation services including RNAi.

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Find the most appropriate screen to accelerate your drug development: discover in vivo screens with MuScreen™ and in vitro cell line screening with OmniScreen™.

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Global CRO in California, USA offering preclinical and translational oncology platforms with high-quality in vivo, in vitro, and ex vivo models.

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Poster 1016: Investigating the TME through PDX RNAseq Analysis

Transcriptomic Analysis of Bulk Tissues from a Large PDX Collection as a Novel Platform for Discovering New TME Targets/Drugs

Jia Xue, Wubin Qian, Sheng Guo, Annie An, Davy Ouyang, and Henry Q. Li

CrownBio 2018. Poster 1016: Investigating the TME through PDX RNAseq AnalysisCancers are collections of diverse diseases, of both genetic and immunological abnormalities. The heterogeneous tumor microenvironment (which includes immune components) and its interaction with tumor cells plays a critical role in tumor progression and in response to anticancer agents, particularly immunotherapies.

However, investigating TME-specific components is particularly challenging due to the difficulty in separating stroma from tumor cells, either physically via microdissection or in silico via bioinformatics.

This poster investigates patient-derived xenograft (PDX) models as a new system to investigate the TME, where human and mouse content can readily be separated in silico. Approximately 1600 bulk tumor tissues from PDX grown in athymic mice have been sequenced for this analysis.

Read this Poster to Discover:

  • The identification of all types of adaptive and innate immune cells in mouse stroma and a variety of human immune components, with corresponding fractions varying across cancer subtypes and individual models
  • The large number of intra-species interactions and a smaller number of inter-species interactions identified by co-regulation analysis, and the reverse correlation between number of interactions and percent take rate across PDX collections
  • A potential role for KRAS mutation in tumor growth independency on TME, demonstrated by highest take rate/lowest interaction number in pancreatic cancer (which has high KRAS mutation rate), and confirmed in KRAS mutant CRC vs wild type

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