ENA 2022 Poster 324

Quantitative Analysis of EGFR Expression and Distribution Upon Drug Treatment in Colorectal Organoid Models

Marrit Putker, Jara Garcia Mateos, Cinthya Del Angel Zuvirie, Leo S. Price, and Bram Herpers

Human organoids are patient-derived tissues that can be expanded in vitro by stimulating the (cancer) stem cells with WNT pathway activators, treatment with differentiation inhibitors and by providing growth factors in an extracellular matrix environment.

These organoids form three-dimensional multicellular structures that polarize and self-organize and express relevant disease-related markers, which makes an organoid biobank ideal for testing the efficacy and mode of action of compounds and antibodies across a diverse patient population. Here, we employed our colorectal organoid biobank and high content imaging platform to characterize EGFR dynamics upon drug treatment.

Download this Poster to Discover:

• How a 3D organoid screening and image analysis platform allows in-depth detection and distinction of growth-factor responses that not necessarily only relate to proliferation.
  
  
• Explore the sensitivity of organoid models to EGF stimulation that are independent of EGF for their outgrowth.
  
  
• How the high content imaging platform in combination with large scale screening in 3D-cultured organoid models allows compound and antibody testing in physiologically relevant patient-derived systems.

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