CICON 2025 Poster 29

Analyzing Bispecific Antibody Effects in Co-cultures of Patient Derived Organoids and Immune Cells with High-content, Image Based Screening Platform

Authors: Sergei Chavez Abiega, Saskia de Man, Tomas Veenendaal, Michelle Kop, Valeria Teodosieva, Jarmil Hanrath, Virgil Woerdings, Emma Spanjaard, Marrit Putker, Gera Goverse

Discover how high-content imaging (HCI) enables functional analysis of bispecific antibody activity across organoids and ex vivo patient material.

Bispecific antibodies are rapidly emerging as powerful immunotherapies, but evaluating their activity in physiologically relevant models remains a challenge. At Crown Bioscience, we have developed a high-content, image-based screening platform to study the complex interactions between immune cells and 3D patient-derived organoids (PDOs).

This poster showcases how our platform captures and quantifies immune cell activation, infiltration, migration, and organoid killing in response to bispecific antibodies targeting Claudin 18.2 and EGFR across multiple tumor types and ex vivo patient samples.

What You’ll Learn

• How high-content imaging enhances bispecific antibody evaluation: Gain insights into 3D organoid co-culture systems and their ability to provide reproducible, spatially resolved functional readouts.
  
  
• Key immune events measured in co-cultures: See how immune activation, migration, infiltration, and tumor killing are captured in real time.
  
  
• Target-specific activity of bispecific antibodies: Explore how Claudin 18.2 and EGFR T cell engagers induced differential effects depending on target expression levels across PDOs.
  
  
• Application to ex vivo patient tumor samples: Understand how this platform extends beyond organoids to directly evaluate patient material from NSCLC samples, providing translational insights.
  
  
• Complementary biomarker analysis: Learn how combining cytokine profiling (e.g., IFNγ) with imaging improves interpretation of immune responses.

Explore the full dataset, figures, and methodology behind our high-content organoid co-culture platform.

Download the Poster Now!