Application Note

Flow Cytometric Analysis of Tumor Infiltrating Lymphocytes (TILs) in Human Cancer Tissue

Flow cytometry is a single cell analytical tool used to characterize immune cell phenotypes. With multiparameter flow cytometry, it creates detailed analysis of cell subsets possible for all types of immuno-oncology studies. Our procedures and ability to analyze tumor infiltrating lymphocytes (TILs) via flow cytometry in high-quality human cancer tissue samples helps prepare customized results.

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• Preparation of a single cell suspension from a human NSCLC patient tissue sample, cell viability was determined via pre-gating of Hoechst positive events followed by Propidium Iodide (PI) staining of cells. The viability of individual cases differs and usually varies between 70 and 95%.
  
  
• The results of preparing a single cell suspension from a human CRC patient tissue sample, CD45 positive cells were selected via the magnetic-based, column-free method by Stemcell Technologies. The purity of the CD45 positive and negative fraction was analyzed via flow cytometry.

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