ANE 2023 Poster C047

Comprehensive Curation of Label-free Proteomics Data for 186 Patient-Derived Xenograft (PDX) Models in HuBase™ Cancer Model Database

Hengyuan Liu , Xiaobo Chen, Sheng Guo, Binchen Mao

Crown Bioscience Inc., 218 Xinghu Street, Suzhou, Jiangsu, 215000, China

In preclinical oncology, the authenticity of data from xenograft models is crucial. Yet, proteomics analysis faced hurdles due to mouse cell contamination and the overlap of human and mouse proteins. Crown Bioscience's HuBase™, with its vast array of PDX models, introduces the separate-then-run protocol, streamlining proteomics profiling and ensuring a more accurate database.

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• The separate-then-run protocol's solution to longstanding proteomics challenges.
  
  
• The methodology of mouse cell removal and the prowess of 4D-DIA technology with LC-MS/MS.
  
  
• The first PDX proteomics dataset without mouse contamination, unlocking new potentials in model selection and biomarker discovery.

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