AACR 2025 Poster 7274

A Preclinical In Vitro Platform for Evaluating the Potency of Bispecific T Cell Engager in 2D and 3D Models

Xuefei Yan, Shiqi Guo, Saskia De Man, Yifan Chen, Jiawei Meng, Michelle Kop, Valeria Teodosieva, Haijuan Yu, Gera Goverse, Jun Zhou, Peng Wang

Bispecific T-cell engagers (BiTEs) are innovative cancer therapeutics designed to harness the immune system by linking T cells to cancer cells, inducing tumor-specific cytotoxicity, which enhances specificity and reduces off-target effects on normal tissue. In this study in vitro assays were established to evaluate the tumor cell-killing and T cell activation of BiTEs.

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• Functional in vitro assays to assess BiTe efficacy in cell lines and organoids
  
  
• How organoids can mimic the tumor microenvironment and represent patient diversity
  
  
• Our complementary assays to unravel all the insights into compound efficacy and MoA including high content imaging, cytokine analysis, flow cytometry and much more

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