Flow-Based Phenotyping of Tumor-Infiltrating Immune Cells in Different I/O Tumor Models
Annie Xiaoyu An, Lily Tong, Davy Ouyang, Dan Duan, Jinping Liu, Tingting Li, Lei Liu, Ling Xu, Ying Jin, Wenqing Yang, Xin Dong*, Jay Liu*, and Henry Q.X. Li
*Nanjing Galaxy Biopharmaceutical Co. Ltd., Nanjing
Immunotherapies such as checkpoint inhibitors have achieved great success in treating cancer patients clinically. However, not all patients respond to these agents, most likely due to patients’ and/or tumors’ genetic and immunological makeup, particularly of the tumor microenvironment (TME).
The connections between specific immune compartments of the TME with response to immunotherapy remain to be elucidated, and preclinical animal models could potentially be highly useful for this research.
CrownBio has developed various novel immuno-oncology models and this poster covers the investigation into model TME, and its relationship with immunotherapy treatment outcomes.
Read this Poster to Discover:
- The development and evaluation of three important immuno-oncology model types: GEMM tumor-derived homografts, syngeneic tumors within mice with specific knock in humanized drug targets, and PDX in CD34+ HSC humanized mice.
- Novel methods to effectively dissect and delineate detailed immunophenotypes within the TME using flow cytometry, including specially customized dissociation for each tumor type, large 16-color panel design, and unique gating strategies.
- The comprehensive profiling of tumor-infiltrating immune cells of a large panel of mouse homograft tumors using multi-color flow cytometry.