Simon Jiang, Amanda Miles*, Carles Puig-Saenz*, Sarah Wagner, Bryan Miller, Yanrui Song, Jason Davies, Annie An, Rajendra Kumari, Davy Ouyang, Henry Qixiang Li, Yinfei Yin
*John van Geest Cancer Research Centre, Nottingham Trent University, Clifton Lane, Clifton, Nottingham, NG11 8NS
Clinically relevant preclinical models recapitulating the human tumor microenvironment (TME) are critical for the successful outcome of immunotherapy studies. The dynamic interplay of cells within the TME drive alterations in cellular functions and impact study outcomes.
Orthotopic syngeneic models are implanted in a relevant organ-specific location to recapitulate the immune and stromal component interactions with the tumor. Orthotopic implantations such as these can be monitored by bioluminescent imaging (BLI).
This poster details the development of a panel of bioluminescent, orthotopic syngeneic models for the enhanced assessment of immunotherapeutics.