AACR20 Poster 2788: Orthotopic, Bioluminescent Syngeneic Models for I/O Evaluation

Characterization of a Panel of Orthotopic Syngeneic Models using Bioluminescent Imaging for the Evaluation of Immuno-Oncology Therapies

Simon Jiang, Amanda Miles*, Carles Puig-Saenz*, Sarah Wagner, Bryan Miller, Yanrui Song, Jason Davies, Annie An, Rajendra Kumari, Davy Ouyang, Henry Qixiang Li, Yinfei Yin
*John van Geest Cancer Research Centre, Nottingham Trent University, Clifton Lane, Clifton, Nottingham, NG11 8NS

Clinically relevant preclinical models recapitulating the human tumor microenvironment (TME) are critical for the successful outcome of immunotherapy studies. The dynamic interplay of cells within the TME drive alterations in cellular functions and impact study outcomes.

Orthotopic syngeneic models are implanted in a relevant organ-specific location to recapitulate the immune and stromal component interactions with the tumor. Orthotopic implantations such as these can be monitored by bioluminescent imaging (BLI).

This poster details the development of a panel of bioluminescent, orthotopic syngeneic models for the enhanced assessment of immunotherapeutics.

Watch this Poster to Discover:

• A panel of bioluminescent, orthotopic syngeneic models, which provide a valuable tool for testing immunotherapies, with a more clinically relevant TME in comparison to the corresponding subcutaneous model
  
  
• How the response to checkpoint inhibitors varies for certain syngeneic models, dependent on subcutaneous vs orthotopic implantation
  
  
• That qualitative proteomic analysis of Pan02 tumors reveals >400 significantly regulated proteins that clearly clustered the replicates according to implantation site

Watch the Poster Now!