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AACR 2022 Poster 1660

Comparison Between surgical and ultrasound-guided orthotopic implantation of hepatic tumour models in mice using ultrasound and bioluminescence imaging

Chris Payne, Yasmin Amer, Cathrine Beatty, Ganisha Hutchinson, Mingfa Zhang, Lucy Harris, Amanda Miles, Ruth Storer, Yinfei Yin

Orthotopic tumor mouse models are often preferred to subcutaneous models due to their increased clinical relevance. However, orthotopic implantation of some cell lines can be time consuming and inefficient due to the surgical procedures involved to establish the tumor.

This study assessed the benefits of replacing traditional invasive surgical orthotopic implantation of hepatic tumor cell lines with a minimally invasive ultrasound guided approach. Tumor growth of both implantation methods were assessed using ultrasound and bioluminescence, as well as duration of surgery and animal recovery time. In addition, the immune landscape of both implantation methods was assessed using flow cytometry.

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  • That ultrasound guided implantation of orthotopic hepatic tumors is a minimally invasive technique that leads to improved recovery times for animals, which adheres to the principles of the 3R’s (reduce, refine, replace)

  • The time taken for ultrasound guided implantation compared to traditional surgical methods was also significantly reduced, enabling a higher throughput of animals on study

  • How tumor growth kinetics for the surgical and ultrasound-guided injection groups were comparable for Hepa 1-6 cells but significantly different for Hep3B cells

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