Transcriptomic Analysis of Patient-Derived Xenografts Reveals Heterogeneity in Human and Mouse Stroma/Immune Compartments
It is being increasingly recognized that cancers are not just genetic diseases, but are also diseases of immunological abnormalities. Research has therefore focused on both cancer genomics as well as immunogenomics, mainly on the tumor microenvironment which can play a critical role in tumor progression and response.
Studying stroma-specific components has been historically challenging; however, newer experimental animal models provide excellent systems for investigating the TME, for example patient-derived xenograft (PDX) models, which closely mirror human disease for a range of factors.
PDX models allow straightforward in silico separation of human and mouse content, providing a surrogate model for TME and tumor-stroma interaction studies. This presentation covers the initial work on the analysis of PDX TME, including whole transcriptome sequencing of almost 1,500 PDX models.
Watch this Presentation to Discover:
- That PDX models have a mouse TME, and that the efficient separation of human vs. murine TME components in silico, along with largely loss of human TME, enables investigation into the relationship between tumor cells and tumor stromal components.
- That there are cross-species interactions between human tumor cells and murine stroma, with different cancers having different types and/or degrees of interaction, implicating relevant heterogeneous biological processes essential for tumor growth.
- How PDX seem to also maintain human immune components, heterogeneous among types, even in later passages, which is now having the full biological meaning being elucidated.