This mouse IgG3 kappa isotype control antibody can be used as a negative control, standard, blocking agent or coating protein in various scientific applications such as ELISA, Western blot, immunofluorescence (IF), immunohistochemistry (IHC), immunoprecipitation (IP), and flow cytometry (FACS). This product is also suitable for various in vitro and in vivo studies which require low endotoxin level since it was produced under stringent endotoxin control conditions.
SEC-HPLC (0.5 ml/min; 30 min; UV280) analysis of Rat IgG1, kappa Isotype Control, catalog # C0010.
SDS-PAGE analysis of Rat IgG1, kappa Isotype Control, catalog # C0010, under reducing and non-reducing conditions.
|Ig subclass:||Rat IgG1, kappa|
|Immunogen:||HEL (Hen Egg Lysozyme)|
|Applications:||WB, ELISA, IF, IHC, IP, FACS, in vivo studies|
|QC:||Endotoxin level: <0.5 EU/mg by the LAL method / Purity: >95% by HPLC and SDS-PAGE|
|Purification Method:||Affinity purified|
|Buffer:||PBS or lyophilized|
|Storage & Handling|
|Storage Conditions:||Liquid: Store at –80° C / Lyophilized: Store at –20° C.
Stable for 12 months from date of purchase when stored as recommended.