Crown Bioscience has extensive experience developing, characterizing and expressing monoclonal antibodies. While specializing in antibodies for early discovery research and in vivo preclinical applications, we can also accommodate antibody development for in vitro diagnostics.
In Vivo GradeAntibodies
Many custom preclinical research projects on our platform have culminated in commercially available in vivo-grade antibodies, now offered in our CrownVivoTM and CrownVivo PremiumTM primary antibody collections. Many have been designed for use in detection, neutralization, and in vivo depletion assays.
Our most popular host animals are mice and hamsters, with hamsters having the unique advantage of being used in vivo in mouse models without eliciting an immune response against the injected antibody.
In Vitro Monoclonal Antibody & Protein Production
Our antibody and protein production platform scales with your research program, supporting projects from proof-of-concept through commercial-scale bioreactor production.
With extensive experience in large scale mammalian cell culture of hybridoma and mammalian cells for production of monoclonal antibodies, Crown is able to support large scale antibody and protein production requests.
Rapid Expression of Human Monoclonal Antibody Variants and Recombinant Proteins
To support early discovery and preclinical research, Crown Bioscience developed a platform of rapid expression services delivering antibodies and proteins of the highest purity available.
- Site-directed mutagenesis to create variant candidates for characterization of potential therapeutic proteins.
- Highly-purified recombinant proteins and antibodies.
- Low-endotoxin functional formulations for preclinical trials.
- Efficient mammalian transgene expression.
Our transgene expression system supports expedited expression of recombinant proteins and monoclonal antibodies for use in early discovery research, preclinical trials, or in vitro diagnostic procedures. It was developed to transfect mammalian cells (CHO or HEK-293) with high efficiency during suspension cell culture in stirred tank bioreactors.
Preparation of F(ab') or F(ab')2 Fragments of Antibodies
Crown’s custom antibody platform supports enzyme digestion of antibodies into univalent F(ab') or F(ab')2 fragments for immunoassays, immunohistochemistry, and in vivo functional assays. Antibody fragments may be adsorbed against serum proteins to remove undesired species cross-reactivity and conjugated to the required reporter molecule.