One-Stop Solutions for Antibody and Protein Services: CrownBio Specializes in the Custom Solutions You Need to Accelerate Your Research
Crown Bioscience offers a broad platform of protein services, ranging from monoclonal antibody development, large scale protein production & purification, and custom antibody conjugations. Our core capabilities include:
- Monoclonal Antibody Development
- Large Scale In Vitro Antibody & Protein Production
- Rapid Expression of Antibodies & Proteins
- Antibody & Protein Purification
- Custom Antibody Conjugations
Monoclonal Antibody Development
Crown Bioscience has extensive experience developing, characterizing and expressing monoclonal antibodies. Many custom projects for our preclinical clients have culminated in commercially available in vivo grade antibodies that are now offered as part of our Life Science CrownVivoTM and CrownVivoPremiumTM primary antibody collections. Many have been designed for use in detection, neutralization, and in vivo depletion assays. Our most popular host animals are mice and hamsters, with hamsters having the unique advantage of being used in vivo in mouse models without eliciting an immune response against the injected antibody. While specializing in antibodies for early discovery research and in vivo preclinical applications, we can also accommodate antibody development for in vitro diagnostics.
Large Scale In Vitro Monoclonal Antibody and Protein Production
With extensive experience in large scale mammalian cell culture of hybridoma and mammalian cells for production of monoclonal antibodies, Crown is able to support large scale antibody and protein production requests. In cases where projects are still in the proof of concept stage, in vitro production can be performed in roller bottles or shake flasks. Bioreactor production is available at small and large scale.
Rapid Expression of Human Monoclonal Antibody Variants and Recombinant Proteins
Essential in the process of understanding mechanisms of disease, preclinical research enables researchers to create models of disease states and elucidate the biochemical and cellular processes responsible for the development of diseases. We are pleased to offer a range of services to scientists requiring specific antibodies and proteins of the highest purity available for conducting early discovery and preclinical research, including:
- Site directed mutagenesis to create variant candidates for characterization of potential therapeutic proteins
- Bio-analytical testing resulting in low endotoxin functional formulations for use in preclinical trials.
- Mammalian transgene expression
The transgene expression system used by Crown has been developed to expedite expression of recombinant proteins and monoclonal antibodies for use in early discovery research, preclinical trials or in vitro diagnostic procedures. It was developed to transfect mammalian cells (CHO or HEK-293) with high efficiency during suspension cell culture in stirred tank bioreactors.
Antibody and Protein Purification
With robust multi-step purification procedures, Crown is able to provide highly purified recombinant proteins and antibodies for use in early discovery research and preclinical trials. A variety of scales from laboratory to commercial manufacturing are available. Development of a purification procedure is based on the final product application and predetermined specifications. In most antibody cases, a robust procedure beginning with protein A chromatography followed by other proprietary chromatography steps result in a highly pure antibody preparation. For in vivo functional studies or preclinical trial materials, procedures resulting in preparations free of aggregates, DNA, viruses, leached protein A and low endotoxins are available.
Representative Chromatography Options Available:
- Protein A affinity
- Protein G affinity
- Ion exchange
- Antigen affinity
- Hydrophobic interaction
- Size Exclusion Chromatography (SEC)
Bio-analytical Lot Release Testing Offered:
- SDS Page analysis for purity
- FPLC analysis for purity and % aggregates
- Isoelectric Profiling (IEF) for identity
- Concentration determination
- Protein A / antibody ratio
- Mycoplasma testing
- Endotoxin testing
- Biological activity
- pH determination
- Freeze thaw recovery
Choose from multiple antibody conjugates for use in applications including flow cytometry, ELISA, Western blotting, immunohistochemistry and membrane assays. See below for a list of common reporter molecules used to tag antibodies for both research and diagnostic applications:
|Reporter Molecule||MW (g/mol)
(x 10 -3)
|ε (M -1cm-1))||Em. Max.
Note: Depending upon excitation and emission requirements, a variety of other reporter molecules are available to be covalently linked to antibodies or proteins.
Final Fill Finish
Conjugates are formulated in stability buffers that increase product shelf life and optimize performance. Final products may be packaged in vials ready for immediate use.
Preparation of F(ab') or F(ab')2 Fragments of Antibodies
We can also support enzyme digestion of antibodies into univalent F(ab') or F(ab')2 fragments for use in immunoassays, immunohistochemistry and in vivo functional assays. Antibody fragments may be adsorbed against serum proteins to remove undesired species cross-reactivity and conjugated to the required reporter molecule.