RANKL Cell Line

Catalog #C2013

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Description

Stable CHO-S clone expressing exogenous human RANKL isoform 1.

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Characterization of RANKL overexpressed in CHO stable clones by FACS.

Product Details

Cell line ID: CHO (RANKL)
Species: Hamster
Cell type: Chinese hamster ovary cell
Gene ID/Accession #: NM_003701.3
Selection marker: Neomycin
Application: Antibody screening and biological assays
Morphology: Epithelial cells in adhesion
Medium: Freestyle CHO-S medium + 2.5% FBS + 750ug/ml G418
Subculture: Split saturated culture 1:10 every 3 days; seed out at about 1-3 x 105 cells/ml
Incubation: 37 °C with 8% CO2
Storage: Frozen in liquid nitrogen with 70% medium, 20% FBS and 10% DMSO
Doubling time: Approximately 48 hours
Biosafety level: 1
References: Cell 93:165-176 (1998)

Resuscitation Protocol

Thaw the vial as soon as possible upon receipt to insure its viability.

  1. Thaw the frozen vial in a 37°C water bath for 1-2 minutes.
  2. Transfer the vial into biosafety cabinet. Wipe the surface with 70% ethanol and drop the cell suspension gently into a centrifuge tube containing 9.0 mL complete culture medium.
  3. Spin at ~ 125 x g, for 5- 7 minutes at room temperature.
  4. Resuspend cell pellet with the appropriate volume of complete medium and transfer the cell suspension into a T25 culture flask.
  5. Incubate the culture at 37°, 8% CO2 incubator or refer to the specific culture condition, if possible.

1 Vial (1M Cells) - $3,235

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