EML4-ALK F1174L Cell Line

Catalog #C2002



Stable Ba/F3 clone expressing exogenous EML4-ALK fusion gene with F1174L point mutation in the ALK part.



Characterization of EML4-ALK Fusion Protein Expression in Different EML4-ALK Stable Cell lines by Western Blot. Protein Lysates from NCI-H2228 Cells Harboring EML4-ALK Fusion, and KARPAS-299 Cells Harboring NPM1-ALK Fusion, were Compared Side by Side in the Same Blot. Negative Control: Parental Ba/F3 Cell Line Extract

Product Details

Cell line ID: Ba/F3 (EML4-ALKF1174L)
Species: Mouse
Cell type: Pro-B cells
Gene ID/Accession #: -
Selection marker: Blasticidin S (2µg/ml)
Application: Drug screening and biological assays
Morphology: Mostly single, round (some polymorph) cells in suspension
Medium: RPMI 1640 + 10% FBS + 2µg/ml Blasticidine
Subculture: Split saturated culture 1:10 every 3 days; seed out at about 1-3 x 105 cells/ml
Incubation: 37 °C with 5% CO2
Storage: Frozen in liquid nitrogen with 70% medium, 20% FBS and 10% DMSO
Doubling time: Approximately 20 hours
Biosafety level: 1
References: Nature Reviews Cancer 2013; 13: 685-700

Resuscitation Protocol

Thaw the vial as soon as possible upon receipt to insure its viability.

  1. Thaw the frozen vial in a 37°C water bath for 1-2 minutes.
  2. Transfer the vial into biosafety cabinet. Wipe the surface with 70% ethanol and drop the cell suspension gently into a centrifuge tube containing 9.0 mL complete culture medium.
  3. Spin at ~ 125 x g, for 5- 7 minutes at room temperature.
  4. Resuspend cell pellet with the appropriate volume of complete medium and transfer the cell suspension into a T25 culture flask.
  5. Incubate the culture at 37°, 5% CO2 incubator or refer to the specific culture condition, if possible.

1 Vial (1M Cells) - $3,235.00

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