EGFR-H773_V774insNPH Cell Line

Catalog #C2058



Stable Ba/F3 clone expressing exogenous EGFR isoform a bearing H773_V774insNPH mutation.



Kinase inhibitor screening using EGFR-H773_V774insNPH cell line, catalog # C2058.

Characterization of EGFR mutant overexpression in Ba/F3 stable clone using FACS.

Product Details

Cell line ID: Ba/F3(EGFR-H773_V774insNPH)
Species: Mouse
Cell type: Pro-B cells
Gene ID/Accession #: NM_005228.3
Selection marker: Puromycin
Application: Drug screening and biological assays
Morphology: Mostly single, round (some polymorph) cells in suspension
Medium: RPMI 1640 + 10% FBS
Subculture: Split saturated culture 1:10 every 3 days; seed out at about 1-3 x 105 cells/ml
Incubation: 37 °C with 5% CO2
Storage: Frozen in liquid nitrogen with 70% medium, 20% FBS and 10% DMSO
Doubling time: Approximately 20 hours
Biosafety level: 1
References: Nature 309:418-425(1984)

Resuscitation Protocol

Thaw the vial as soon as possible upon receipt to insure its viability.

  1. Thaw the frozen vial in a 37°C water bath for 1-2 minutes.
  2. Transfer the vial into biosafety cabinet. Wipe the surface with 70% ethanol and drop the cell suspension gently into a centrifuge tube containing 9.0 mL complete culture medium.
  3. Spin at ~ 125 x g, for 5- 7 minutes at room temperature.
  4. Resuspend cell pellet with the appropriate volume of complete medium and transfer the cell suspension into a T25 culture flask.
  5. Incubate the culture at 37°, 5% CO2 incubator or refer to the specific culture condition, if possible.

1 Vial (1M Cells) - $3,235

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