CD48 Recombinant Cell Line

Drug Screening and Biological Assays
Gene of Interest: CD48

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CD48 Cell Line Background

CD48, also referred to as B-lymphocyte activation marker (BLAST-1) or signaling lymphocytic activation molecule (SLAMF2), is membrane protein of immunoglobulin superfamily. CD48 is expressed on peripheral blood lymphocytes and participates in the activation and differention of immune cells.

Alternative Nomenclature

The CD48 molecule (CD48) is also known as BCM1, BLAST, hCD48, mCD48, BLAST1, SLAMF2, or MEM-102.

Recombinant Cell Line Generation

This 293T human CD48 cell line was generated using pLVX-puro vector with human CD48 gene sequence.

Cell Line Characterization

FACs analysis of human CD48 recombinant cell line
FACS analysis of cell surface CD48 expression by an anti-hCD48 antibody and isotype control antibody.

Recombinant Cell Line Profile

Cell line ID: 293T/CD48
Species: Human
Cell type: Human embryonic kidney
Gene ID/Accession #: NM_001256030.1
Selection marker: Puromycin (0.5ug/ml)
Application: Drug screening and biological assays
Morphology: Fibroblastoid cells growing adherently as monolayer
Medium: DMEM + 10% FBS
Subculture: Seed out at ca. 2-3 x 106 cells/80 cm2; split confluent culture 1:4 - 1:5 every 2-3 days
Incubation: 37 °C with 5% CO2
Storage: Frozen in liquid nitrogen with 70% medium, 20% FBS and 10% DMSO
Doubling time: 24-30 hours
Reporter gene: Firefly luciferase
Biosafety level: 1
References: Immunogenetics 33:113-117(1991)

Resuscitation Protocol

Thaw the vial as soon as possible upon receipt to insure its viability.

  1. Thaw the frozen vial in a 37°C water bath for 1-2 minutes.
  2. Transfer the vial into biosafety cabinet. Wipe the surface with 70% ethanol and drop the cell suspension gently into a centrifuge tube containing 9.0 mL complete culture medium.
  3. Spin at ~ 125 x g, for 5- 7 minutes at room temperature.
  4. Resuspend cell pellet with the appropriate volume of complete medium and transfer the cell suspension into a T25 culture flask.
  5. Incubate the culture at 37°, 5% CO2 incubator or refer to the specific culture condition, if possible.

1 Vial (1M Cells) - $3,235

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