CD224 Recombinant Cell Line

Drug Screening and Biological Assays
Gene of Interest: CD224

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CD224 Cell Line Background

CD244, also referred to as Natural Killer (NK) cell receptor 2B4, is a surface receptor expressed on NK cells and some T cells which functions as a modulator of cytolytic activity through interaction with MHC molecules.

Alternative Nomenclature

The CD244 molecule (CD224) is also known as 2B4, NAIL, Nmrk, NKR2B4, or SLAMF4.

Recombinant Cell Line Generation

This 293T human CD244 cell line was generated using pLVX-puro vector with human CD244 gene sequence.

Cell Line Characterization

FACs analysis of human CD244 recombinant cell line
FACS analysis of cell surface CD244 expression by an anti-human CD244 antibody and isotype control antibody.

Recombinant Cell Line Profile

Cell line ID: 293T/CD244
Species: Human
Cell type: Human embryonic kidney
Gene ID/Accession #: NM_016382.3
Selection marker: Puromycin (0.5ug/ml)
Application: Drug screening and biological assays
Morphology: Fibroblastoid cells growing adherently as monolayer
Medium: DMEM + 10% FBS
Subculture: Seed out at ca. 2-3 x 106 cells/80 cm2; split confluent culture 1:4 - 1:5 every 2-3 days
Incubation: 37 °C with 5% CO2
Storage: Frozen in liquid nitrogen with 70% medium, 20% FBS and 10% DMSO
Doubling time: 24-30 hours
Biosafety level: 1
References: Eur. J. Immunol. 29:3466-3477(1999)

Resuscitation Protocol

Thaw the vial as soon as possible upon receipt to insure its viability.

  1. Thaw the frozen vial in a 37°C water bath for 1-2 minutes.
  2. Transfer the vial into biosafety cabinet. Wipe the surface with 70% ethanol and drop the cell suspension gently into a centrifuge tube containing 9.0 mL complete culture medium.
  3. Spin at ~ 125 x g, for 5- 7 minutes at room temperature.
  4. Resuspend cell pellet with the appropriate volume of complete medium and transfer the cell suspension into a T25 culture flask.
  5. Incubate the culture at 37°, 5% CO2 incubator or refer to the specific culture condition, if possible.

1 Vial (1M Cells) - $3,235

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