CD160 Recombinant Cell Line

Drug Screening and Biological Assays
Gene of Interest: CD160



CD160 Cell Line Background

CD160, a glycosylphosphatidylinositol-anchored member of the immunoglobulin superfamily, is expressed on peripheral blood NK cells and CD8+ T lymphocytes with cytolytic activity.

Alternative Nomenclature

The CD160 molecule (CD160) is also known as NK1, BY55, or NK28.

Recombinant Cell Line Generation

This 293T human CD160 cell line was generated using pLVX-puro vector with human CD160 gene sequence.

Cell Line Characterization

FACs analysis of human CD160 recombinant cell line
FACS analysis of cell surface CD160 expression by an anti-human CD160 antibody and isotype control antibody

Recombinant Cell Line Profile

Cell line ID: 293T/CD160
Species: Human
Cell type: Human embryonic kidney
Gene ID/Accession #: NM_007053.3
Selection marker: Puromycin (0.5ug/ml)
Application: Drug screening and biological assays
Morphology: Fibroblastoid cells growing adherently as monolayer
Medium: DMEM + 10% FBS
Subculture: Seed out at ca. 2-3 x 106 cells/80 cm2; split confluent culture 1:4 - 1:5 every 2-3 days
Incubation: 37 °C with 5% CO2
Storage: Frozen in liquid nitrogen with 70% medium, 20% FBS and 10% DMSO
Doubling time: 24-30 hours
Biosafety level: 1
References: J. Immunol. 161:2780-2790(1998)

Resuscitation Protocol

Thaw the vial as soon as possible upon receipt to insure its viability.

  1. Thaw the frozen vial in a 37°C water bath for 1-2 minutes.
  2. Transfer the vial into biosafety cabinet. Wipe the surface with 70% ethanol and drop the cell suspension gently into a centrifuge tube containing 9.0 mL complete culture medium.
  3. Spin at ~ 125 x g, for 5- 7 minutes at room temperature.
  4. Resuspend cell pellet with the appropriate volume of complete medium and transfer the cell suspension into a T25 culture flask.
  5. Incubate the culture at 37°, 5% CO2 incubator or refer to the specific culture condition, if possible.

1 Vial (1M Cells) - $3,235

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