B7X Cell Line (Monkey)

Catalog # C2074

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B7X Cell Line Background

B7X belongs to the B7 costimulatory protein family. Proteins in this family are present on the surface of antigen-presenting cells and interact with ligand bound to receptors on the surface of T cells. Studies have shown that high levels of the encoded protein has been correlated with tumor progression. A pseudogene of this gene is located on chromosome 20. Multiple transcript variants encoding different isoforms have been found for this gene.

Cell Line Generation

293T Cynomolgus monkey B7X cell Line was generated using lentiviral vector expressing monkey B7X plus flag sequence.

Cell Line Characterization

Characterization of cynomolgus monkey B7X overexpression in 293T stable clones by FACS.
Characterization of cynomolgus monkey B7X overexpression in 293T stable clones by FACS.

Recombinant Cell Line Profile

Cell line ID: 293T/Monkey B7X
Species: Human
Cell type: Human embryonic kidney
Gene ID/Accession #: XM_015430437.1
Selection marker: Puromycin (0.5ug/ml)
Application: Drug screening and biological assays
Morphology: Fibroblastoid cells growing adherently as monolayer
Medium: DMEM + 10% FBS
Subculture: Seed out at ca. 2-3 x 106 cells/80 cm2; split confluent culture 1:4 - 1:5 every 2-3 days
Incubation: 37 °C with 5% CO2
Storage: Frozen in liquid nitrogen with 70% medium, 20% FBS and 10% DMSO
Doubling time: 24-30 hours
Biosafety level: 1
References: Exp. Cell Res. 306:128-141(2005)

Resuscitation Protocol

Thaw the vial as soon as possible upon receipt to insure its viability.

  1. Thaw the frozen vial in a 37°C water bath for 1-2 minutes.
  2. Transfer the vial into biosafety cabinet. Wipe the surface with 70% ethanol and drop the cell suspension gently into a centrifuge tube containing 9.0 mL complete culture medium.
  3. Spin at ~ 125 x g, for 5- 7 minutes at room temperature.
  4. Resuspend cell pellet with the appropriate volume of complete medium and transfer the cell suspension into a T25 culture flask.
  5. Incubate the culture at 37°, 5% CO2 incubator or refer to the specific culture condition, if possible.

1 Vial (1M Cells) - $3,235

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