p95HER2 Cell Line (T47D)

Catalog # C2069

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p95HER2 Cell Line Background

HER2 is a member of the epidermal growth factor (EGF) receptor family of receptor tyrosine kinases. This protein has no ligand binding domain of its own and therefore cannot bind growth factors. However, it does bind tightly to other ligand-bound EGF receptor family members to form a heterodimer, stabilizing ligand binding and enhancing kinase-mediated activation of downstream signaling pathways, such as those involving mitogen-activated protein kinase and phosphatidylinositol-3 kinase. Amplification and/or overexpression of this gene has been reported in numerous cancers, including breast and ovarian tumors. Alternative splicing results in several additional transcript variants, some encoding different isoforms and others that have not been fully characterized.

Cell Line Generation

T47D p95HER2 cell Line was generated using plasmid expressing p95HER2 sequence.

Cell Line Characterization

Characterization of HER2 and its mutants overexpressed in T47D stable clones using Western Blot.
Characterization of HER2 and its mutants overexpressed in T47D stable clones using Western Blot.

Cell Line Applications

A. Cell-based kinase inhibition screen
B. Cell viability assay
C. In vivo efficacy study

Recombinant Cell Line Profile

Cell line ID: T47D(P95HER2)
Species: Human
Cell type: Human breast cancer cell line
Gene ID/Accession #: NM_004448.3
Selection marker: N/A
Application: Drug screening and biological assays
Morphology: Adherent epithelial cell
Medium: RPMI 1640 + 10% FBS
Subculture: Split saturated culture 1:3 every 3 days; seed out at about 1-3 x 105 cells/ml
Incubation: 37 °C with 5% CO2
Storage: Frozen in liquid nitrogen with 70% medium, 20% FBS and 10% DMSO
Doubling time: N/A
Biosafety level: 1
References: N/A

Resuscitation Protocol

Thaw the vial as soon as possible upon receipt to insure its viability.

  1. Thaw the frozen vial in a 37°C water bath for 1-2 minutes.
  2. Transfer the vial into biosafety cabinet. Wipe the surface with 70% ethanol and drop the cell suspension gently into a centrifuge tube containing 9.0 mL complete culture medium.
  3. Spin at ~ 125 x g, for 5- 7 minutes at room temperature.
  4. Resuspend cell pellet with the appropriate volume of complete medium and transfer the cell suspension into a T25 culture flask.
  5. Incubate the culture at 37°, 5% CO2 incubator or refer to the specific culture condition, if possible.

1 Vial (1M Cells) - $3,235

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