CEACAM-1 Short Isoform Cell Line

Catalog # C2062

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CEACAM-1 Cell Line Background

CEACAM-1 is a member of the carcinoembryonic antigen (CEA) gene family, which belongs to the immunoglobulin superfamily. CEACAM-1 was originally described in bile ducts of liver as biliary glycoprotein. Subsequently, it was found to be a cell-cell adhesion molecule detected on leukocytes, epithelia, and endothelia. The encoded protein mediates cell adhesion via homophilic as well as heterophilic binding to other proteins of the subgroup. Multiple cellular activities have been attributed to the encoded protein, including roles in the differentiation and arrangement of tissue three-dimensional structure, angiogenesis, apoptosis, tumor suppression, metastasis, and the modulation of innate and adaptive immune responses. Multiple transcript variants encoding different isoforms have been reported, but the full-length nature of all variants has not been defined.

Cell Line Generation

293T Human CEMCAM-1 short isoform cell line was generated using lentiviral vector with human CEACAM-1 gene sequence.

Cell Line Characterization

Characterization of CEACAM-1 variants overexpressed in 293T stable clones using Western Blot.
Characterization of CEACAM-1 variants overexpressed in 293T stable clones using Western Blot.

Recombinant Cell Line Profile

Cell line ID: 293T/CEACAM-1 short isoform
Species: Human
Cell type: Human embryonic kidney
Gene ID/Accession #: NM_001184816
Selection marker: Puromycin (0.5ug/ml)
Application: Drug screening and biological assays
Morphology: Fibroblastoid cells growing adherently as monolayer
Medium: DMEM + 10% FBS
Subculture: Seed out at ca. 2-3 x 106 cells/80 cm2; split confluent culture 1:4 - 1:5 every 2-3 days
Incubation: 37 °C with 5% CO2
Storage: Frozen in liquid nitrogen with 70% medium, 20% FBS and 10% DMSO
Doubling time: 24-30 hours
Biosafety level: 1
References: Nature Reviews. Immunology. 6 (6): 433–46.

Resuscitation Protocol

Thaw the vial as soon as possible upon receipt to insure its viability.

  1. Thaw the frozen vial in a 37°C water bath for 1-2 minutes.
  2. Transfer the vial into biosafety cabinet. Wipe the surface with 70% ethanol and drop the cell suspension gently into a centrifuge tube containing 9.0 mL complete culture medium.
  3. Spin at ~ 125 x g, for 5- 7 minutes at room temperature.
  4. Resuspend cell pellet with the appropriate volume of complete medium and transfer the cell suspension into a T25 culture flask.
  5. Incubate the culture at 37°, 5% CO2 incubator or refer to the specific culture condition, if possible.

1 Vial (1M Cells) - $3,235

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