BCR-ABL T315I Cell Line

Catalog #C2008

products.png

Description

Stable Ba/F3 clone expressing exogenous BCR-ABL fusion gene with T315I point mutation in the ABL kinase domain.

ico-pdf.png

Images


Characterization of Drug Sensitivity in BCR-ABL T315I Cell Line, Catalog # C2008. C2008 was Treated with 5 Different Inhibitors for 72 Hours in 96-Well Plates. Cell Viability was Measured using CellTiter-Glo®. Dose Response Curve and IC50s were Fitted and Calculated using GraphPad Prism.

Product Details

Cell line ID: Ba/F3 (BCR-ABLT315I )
Species: Mouse
Cell type: Pro-B cells
Gene ID/Accession #: -
Selection marker: None
Application: Drug screening and biological assays
Morphology: Mostly single, round (some polymorph) cells in suspension
Medium: RPMI 1640 + 10% FBS
Subculture: Split saturated culture 1:10 every 3 days; seed out at about 1-3 x 105 cells/ml
Incubation: 37 °C with 5% CO2
Storage: Frozen in liquid nitrogen with 70% medium, 20% FBS and 10% DMSO
Doubling time: Approximately 20 hours
Biosafety level: 1
References: Science. 2001 Aug 3;293(5531):876-80.

Resuscitation Protocol

Thaw the vial as soon as possible upon receipt to insure its viability.

  1. Thaw the frozen vial in a 37°C water bath for 1-2 minutes.
  2. Transfer the vial into biosafety cabinet. Wipe the surface with 70% ethanol and drop the cell suspension gently into a centrifuge tube containing 9.0 mL complete culture medium.
  3. Spin at ~ 125 x g, for 5- 7 minutes at room temperature.
  4. Resuspend cell pellet with the appropriate volume of complete medium and transfer the cell suspension into a T25 culture flask.
  5. Incubate the culture at 37°, 5% CO2 incubator or refer to the specific culture condition, if possible.

1 Vial (1M Cells) - $3,235

Add to Cart

Academic / Non-Profit Pricing Applied In Cart

Ask a Question / Request a Quote

For large volume quotes, or to ask a question about this product please complete the form below, or call:

+1 855 827 6968
6am - 5pm (PST)