BTLA also known as cd272 is a ligand for tnfrsf14(hevm) and expressed on the activated t-cells functioned negative regulator of cell immune response through btla-hvem complex
Characterization Using FACs (image caption popout?)
FACS analysis of cell surface BTLA expression by an anti-human BTLA antibody and isotype control antibody.
BTLA Cell Line Product Details
|Cell line ID:||293T/BTLA|
|Cell type:||Human embryonic kidney|
|Description:||HEK 293T cells stably expressing exogenous human BTLA gene.|
|Cell Line Generation:||293T Human BTLA Cell Line was generated using pLVX-puro Vector with Human BTLA gene sequence.|
|Gene ID/Accession #:||NM_181780.3|
|Selection marker:||Puromycin (0.5 ug/ml)|
|Application:||Drug screening and biological assays | Hybridoma or binders of BTLA screening with FACS|
|Morphology:||Fibroblastoid cells growing adherently as monolayer|
|Medium:||DMEM + 10% FBS|
|Subculture:||Seed out at ca. 2-3 x 106 cells/80 cm2; split confluent culture 1:4 - 1:5 every 2-3 days|
|Incubation:||37 °C with 5% CO2|
|Storage:||Frozen in liquid nitrogen with 70% medium, 20% FBS and 10% DMSO|
|Doubling time:||24-30 hours|
Nat. Immunol. 4:670-679(2003)
Thaw the vial as soon as possible upon receipt to insure its viability.
- Thaw the frozen vial in a 37°C water bath for 1-2 minutes.
- Transfer the vial into biosafety cabinet. Wipe the surface with 70% ethanol and drop the cell suspension gently into a centrifuge tube containing 9.0 mL complete culture medium.
- Spin at ~ 125 x g, for 5- 7 minutes at room temperature.
- Resuspend cell pellet with the appropriate volume of complete medium and transfer the cell suspension into a T25 culture flask.
- Incubate the culture at 37°, 5% CO2 incubator or refer to the specific culture condition, if possible.
Cell Line Limited Use License
FOR IN VITRO REASEARCH USE ONLY. NOT FOR THERAPEUTIC OR DIAGNOSTIC USE. BY ACCEPTING THIS PRODUCT, RECIPIENT AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE STATEMENT.
This product may not be resold or transferred by the recipient and may be used only by the recipient, in their facility and only for (1) research use, (2) drug discovery and development of biologic drugs, (3) quality assurance testing of biologic drugs, and (4) assays for biologic drugs. No other commercial use is allowed. “Commercial use” means any and all uses of this product by recipient for monetary or other consideration, including providing a service, supplying information or data to unaffiliated third parties, and resale of this product for any use. Recipient has no right to modify, derivatize, genetically engineer or otherwise create variations of the Cells.
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To obtain a COMMERCIAL USE License please contact Crown Bioscience, Inc.