ELISA of three different PEGylated BSAs using PEG antibody (clone RM105), followed by an AP conjugated goat anti-rabbit IgG.
Polyethylene glycol (PEG) is a polyether compound with many applications from industrial manufacturing to medicine. PEG is also known as polyethylene oxide (PEO) or polyoxyethylene (POE), depending on its molecular weight. PEG is used as an excipient in many pharmaceutical products. Lower-molecular-weight variants are used as solvents in oral liquids and soft capsules, whereas solid variants are used as ointment bases, tablet binders, film coatings, and lubricants. Polyethylene glycol is commonly used to fuse B-cells with myeloma cells in monoclonal antibody production (PubChem).
|Immunogen:||KLH-PEG with terminal methoxy group|
|Specificity:||This antibody reacts to the methoxy group of Polyethylene glycol (PEG). It is specific for methoxypolyethylene glycol.|
|Applications:||ELISA, IHC, ICC, WB, IP, FC|
|Recommended dilutions:||ELISA: 0.01ug/mL – 0.3ug/mL
IHC: 0.5ug/mL – 2ug/mL
WB: 0.1ug/mL – 1ug/mL
|Purification Method:||Protein A affinity purified from an animal origin–free and protein-free culture supernatant|
|Buffer:||50% Glycerol/PBS with 1% BSA and 0.09% sodium azide|
|Storage Conditions:||Stable for 1 year at -20.0°C from date of receipt.|